Chromatographia

, Volume 71, Issue 11, pp 1081–1086

Rapid RP-LC Method with Fluorescence Detection for Analysis of Fexofenadine in Human Plasma

Authors

    • Department of Pharmaceutical ChemistryMedical University of Gdańsk
  • Alina Plenis
    • Department of Pharmaceutical ChemistryMedical University of Gdańsk
  • Ilona Olędzka
    • Department of Pharmaceutical ChemistryMedical University of Gdańsk
  • Piotr Kowalski
    • Department of Pharmaceutical ChemistryMedical University of Gdańsk
  • Tomasz Bączek
    • Department of Pharmaceutical ChemistryMedical University of Gdańsk
Full Short Communication

DOI: 10.1365/s10337-010-1592-z

Cite this article as:
Konieczna, L., Plenis, A., Olędzka, I. et al. Chroma (2010) 71: 1081. doi:10.1365/s10337-010-1592-z

Abstract

A rapid and sensitive reversed-phase high-performance liquid chromatographic method for analysis of fexofenadine in human plasma has been developed and optimized. The analytes were extracted from biological samples by solid-phase extraction on hydrophilic–lipophilic balance cartridges. LC separation was performed on a C18 analytical column (125 mm × 4 mm i.d., 5-μm particles) with 42:58 (v/v) acetonitrile–water adjusted to pH 2.7 with 85% orthophosphoric acid as mobile phase. Fluorescence detection was performed with excitation at 230 nm and emission at 290 nm. The total time for chromatographic separation was 7 min. The method was validated in accordance with EU guidelines by analysis of plasma samples fortified with fexofenadine at concentrations between 0.05 and 800 ng mL−1. Calibration plots were linear in this range. Mean recovery was typically 94.03% and the detection limit was 0.05 ng mL−1. The time required for quantitative analysis is shorter than that required by other methods.

Keywords

Column liquid chromatographyFluorescence detectionSolid-phase extractionFexofenadine

Copyright information

© Vieweg+Teubner Verlag | Springer Fachmedien Wiesbaden GmbH 2010