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HPTLC Densitometric Quantification of Glycyrrhizin, Glycyrrhetinic Acid, Apigenin, Kaempferol and Quercetin from Glycyrrhiza glabra

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Abstract

Glycyrrhiza glabra Linn., commonly known as liquorice, is a reputed drug of Ayurveda. In the present work we developed and validated densitometric methods for quantification of glycyrrhizin, glycyrrhetinic acid, apigenin, kaempferol and quercetin using HPTLC. The developed methods were found to be precise and accurate. The amount of glycyrrhizin, glycyrrhetinic acid, and quercetin was found to be 1.070, 0.84 and 0.271% w/w, respectively. Apigenin and kaempferol were quantified in free (0.007 and 0.033% w/w) as well as bound (0.021 and 0.074% w/w) forms. This is the first report of simultaneous quantification of glycyrrhetinic acid and apigenin as well as kaempferol and quercetin from G. glabra. Furthermore, no TLC densitometric methods have been reported for the quantification of apigenin, kaempferol and quercetin from G. glabra.

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Acknowledgment

The authors are thankful to Prof. Harish Padh, Project Director, NIPER-Ahmedabad for providing the infrastructure and facilities. The authors are also thankful to the two anonymous reviewers for their valuable comments, suggestions and critical review of the manuscript.

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Authors and Affiliations

  1. Department of Natural Products, National Institute of Pharmaceutical Education and Research, Ahmedabad, Gujarat, 380054, India

    Baljinder Singh, Pankaj Mungara & Sheetal Anandjiwala

  2. Department of Pharmacology and Toxicology, B. V. Patel PERD Centre, Ahmedabad, Gujarat, 380054, India

    Manish Nivsarkar

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  1. Baljinder Singh
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  2. Pankaj Mungara
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  3. Manish Nivsarkar
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  4. Sheetal Anandjiwala
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Correspondence to Sheetal Anandjiwala.

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Singh, B., Mungara, P., Nivsarkar, M. et al. HPTLC Densitometric Quantification of Glycyrrhizin, Glycyrrhetinic Acid, Apigenin, Kaempferol and Quercetin from Glycyrrhiza glabra . Chroma 70, 1665–1672 (2009). https://doi.org/10.1365/s10337-009-1383-6

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