Chromatographia

, 70:1581

Simultaneous Determination of Clopidogrel and Its Carboxylic Acid Metabolite (SR26334) in Human Plasma by LC–ESI–MS–MS: Application to the Therapeutic Drug Monitoring of Clopidogrel

  • Jian-Jun Zou
  • Hong-Wei Fan
  • Da-Qing Guo
  • Ying-Bin Li
  • Song Lin
  • Yu-Bing Zhu
  • Cui-Xia Yu
  • Jie Zhou
  • Jiang-Hui Liu
  • Yun-Fang Hu
Original

DOI: 10.1365/s10337-009-1349-8

Cite this article as:
Zou, J., Fan, H., Guo, D. et al. Chroma (2009) 70: 1581. doi:10.1365/s10337-009-1349-8

Abstract

A sensitive and specific liquid chromatography-tandem-mass spectrometry method was developed and validated for the simultaneous determination of clopidogrel and its carboxylic acid metabolite (SR26334) in human plasma using nateglinide and pioglitazone as internal standards. Analytes were extracted from 0.50 mL of plasma using diethyl ether–n-hexane (4:1, v/v). Chromatographic separation was performed on a Teknokroma C18 column with a mobile phase of methanol–water (containing 0.1% formic acid) (80:20, v/v) at a flow rate of 0.20 mL min−1 within 5.6 min. Linearity was established over the concentration range of 0.005–5 ng mL−1 for clopidogrel and 20–2,500 ng mL−1 for SR26334. Intra- and inter-batch standard deviations were less than 9.2% and the accuracy of this assay was found to fall within an acceptable range ≤10.0%. The method was successfully applied to the therapeutic drug monitoring of clopidogrel.

Keywords

Column liquid chromatographyTandem mass spectrometryTherapeutic drug monitoringClopidogrelClopidogrel carboxylic acid metabolite SR26334

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Jian-Jun Zou
    • 1
  • Hong-Wei Fan
    • 1
  • Da-Qing Guo
    • 2
  • Ying-Bin Li
    • 3
  • Song Lin
    • 4
  • Yu-Bing Zhu
    • 1
  • Cui-Xia Yu
    • 1
  • Jie Zhou
    • 1
  • Jiang-Hui Liu
    • 1
  • Yun-Fang Hu
    • 1
  1. 1.Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical UniversityNanjingPeople’s Republic of China
  2. 2.Department of Pharmacy, Xiangya HospitalCentral South UniversityChangshaPeople’s Republic of China
  3. 3.Department of Neurosurgery, Nanjing First HospitalNanjing Medical UniversityNanjingPeople’s Republic of China
  4. 4.Department of Cardiology, Nanjing First HospitalNanjing Medical UniversityNanjingPeople’s Republic of China