Chromatographia

, 70:1581

Simultaneous Determination of Clopidogrel and Its Carboxylic Acid Metabolite (SR26334) in Human Plasma by LC–ESI–MS–MS: Application to the Therapeutic Drug Monitoring of Clopidogrel

Authors

  • Jian-Jun Zou
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
  • Da-Qing Guo
    • Department of Pharmacy, Xiangya HospitalCentral South University
  • Ying-Bin Li
    • Department of Neurosurgery, Nanjing First HospitalNanjing Medical University
  • Song Lin
    • Department of Cardiology, Nanjing First HospitalNanjing Medical University
  • Yu-Bing Zhu
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
  • Cui-Xia Yu
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
  • Jie Zhou
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
  • Jiang-Hui Liu
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
  • Yun-Fang Hu
    • Department of Clinical Pharmacology, Nanjing First HospitalNanjing Medical University
Original

DOI: 10.1365/s10337-009-1349-8

Cite this article as:
Zou, J., Fan, H., Guo, D. et al. Chroma (2009) 70: 1581. doi:10.1365/s10337-009-1349-8

Abstract

A sensitive and specific liquid chromatography-tandem-mass spectrometry method was developed and validated for the simultaneous determination of clopidogrel and its carboxylic acid metabolite (SR26334) in human plasma using nateglinide and pioglitazone as internal standards. Analytes were extracted from 0.50 mL of plasma using diethyl ether–n-hexane (4:1, v/v). Chromatographic separation was performed on a Teknokroma C18 column with a mobile phase of methanol–water (containing 0.1% formic acid) (80:20, v/v) at a flow rate of 0.20 mL min−1 within 5.6 min. Linearity was established over the concentration range of 0.005–5 ng mL−1 for clopidogrel and 20–2,500 ng mL−1 for SR26334. Intra- and inter-batch standard deviations were less than 9.2% and the accuracy of this assay was found to fall within an acceptable range ≤10.0%. The method was successfully applied to the therapeutic drug monitoring of clopidogrel.

Keywords

Column liquid chromatographyTandem mass spectrometryTherapeutic drug monitoringClopidogrelClopidogrel carboxylic acid metabolite SR26334

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009