Chromatographia

, 69:1251

LC Method for Analysis of Three Flavonols in Rat Plasma and Urine after Oral Administration of Polygonum aviculare Extract

Original

DOI: 10.1365/s10337-009-1088-x

Cite this article as:
Xu, F., Guan, H., Li, G. et al. Chroma (2009) 69: 1251. doi:10.1365/s10337-009-1088-x

Abstract

A high-performance liquid chromatographic method has been developed for the simultaneous analysis of the flavonols myricitrin (1), avicularin (2), and juglanin (3) in rat plasma and urine after oral administration of the total flavonoids from Polygonum aviculare. Samples were prepared by solid-phase extraction then separated on a C18 reversed-phase column by use of a mobile-phase gradient prepared from methanol and aqueous formic acid solution. The flow rate was 1 mL min−1. Detection was performed at 254 nm. The calibration range was 11–1,100 μg mL−1 for both 2 and 3 in plasma; in urine the calibration ranges for 1, 2, and 3 were 32–1,600, 11–1,100, and 22–1,100 μg mL−1, respectively. Intra-day and inter-day RSD were less than 4.33 and 3.62% for 2 and 3, respectively, in plasma, and no more than 4.03 and 2.22% for all the analytes in urine. The analytical sensitivity and selectivity of the assay enabled successful application to pharmacokinetic studies of flavonols 13 in rats.

Keywords

Column liquid chromatographyFlavonolsPlasmaUrinePolygonum aviculare

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Fuquan Xu
    • 1
  • Huashi Guan
    • 1
  • Guoqiang Li
    • 1
  • Hongbing Liu
    • 1
  1. 1.Key Laboratory of Marine Drugs, Ministry of Education, Marine Drug and Food InstituteOcean University of ChinaQingdaoPeople’s Republic of China