Chromatographia

, Volume 69, Issue 9, pp 1077–1082

LC–UV Assay for Simultaneous Estimation of Aromatic Turmerone, α/β-Turmerone and Curlone: Major Bisabolane Sesquiterpenes of Turmeric Oil in Rabbit Plasma for Application to Pharmacokinetic Studies

  • Nitin Mehrotra
  • Sreedharan Sabarinath
  • Satyendra Suryawanshi
  • Kanwal Raj
  • Ram Chandra Gupta
Full Short Communication

DOI: 10.1365/s10337-009-1007-1

Cite this article as:
Mehrotra, N., Sabarinath, S., Suryawanshi, S. et al. Chroma (2009) 69: 1077. doi:10.1365/s10337-009-1007-1

Abstract

The herbal medicament derived from the lipid soluble fraction obtained from Curcuma longa L. (Zingiberaceae) has shown potential neuroprotective activity in disorders like stroke. HM has been standardized with three biomarkers: ar-turmerone, α/β-turmerone and curlone, major bisabolane sesquiterpenes of turmeric oil. Development of a biaonalytical method for these sesquiterpenes was initiated to characterize its preclinical pharmacokinetics in rabbits to accelerate its development as a potential candidate for vascular complications. Since, the compounds are structurally and chemically very similar, gradient elution was utilized on a C-18 reversed phase column with a mobile phase comprising of acetonitrile and deionised water. The UV detector was set at wavelengths 240 and 270 nm. The sample clean-up was performed by protein precipitation with acetonitrile. The method was reasonably sensitive with limits of quantitation (LOQ) of 0.098 μg mL−1 in plasma for all the analytes. Accuracy and precision were within the acceptable limits, as indicated by relative standard deviation (% RSD) varying from 1.3 to 13.6% and bias values ranging from −5.5 to 10.3%, respectively. Moreover, the analytes were stable in plasma even after three freeze-thaw cycles. The method was applied to generate preliminary pharmacokinetics of turmeric oil in rabbits after intravenous administration.

Keywords

Column liquid chromatography Gradient elution Pharmacokinetics Bisabolane sesquiterpenes Turmeric oil 

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Nitin Mehrotra
    • 1
  • Sreedharan Sabarinath
    • 1
  • Satyendra Suryawanshi
    • 1
  • Kanwal Raj
    • 2
  • Ram Chandra Gupta
    • 1
  1. 1.Division of Pharmacokinetics and MetabolismCentral Drug Research InstituteLucknowIndia
  2. 2.Division of Medicinal and Process ChemistryCentral Drug Research InstituteLucknowIndia

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