Chromatographia

, Volume 69, Supplement 2, pp 189–194

An LC Ion-Pairing Method for the Determination of Fe(II) in Ferrous Bisglycinate Pharmaceutical Formulation

  • Danieli Cátia Ceni
  • Laura Alegria Martins
  • Andréa Garcia Pereira
  • Pedro Eduardo Fröehlich
  • Ana Maria Bergold
Full Short Communication

DOI: 10.1365/s10337-009-1000-8

Cite this article as:
Ceni, D.C., Martins, L.A., Pereira, A.G. et al. Chroma (2009) 69(Suppl 2): 189. doi:10.1365/s10337-009-1000-8

Abstract

A reversed-phase ion-pairing liquid chromatographic method was developed and validated for the assay of Fe(II) in ferrous bisglycinate (Fe-bis-gly) capsules using 4-(2-pyridylazo) resorcinol reagent. The analysis was carried out using a Gemini RP-18 (150 mm × 4.6 mm I.D., particle size 5 μm) analytical column; the mobile phase consisted of a mixture of acetonitrile–water (28:72 v/v) containing 1 mM tetrabutylammonium hydrogensulfate and 1% phosphate buffer (pH 8.0). The flow rate was 1.0 mL min−1 and the detection was achieved with a photodiode array (PDA) detector at 706 nm. The specificity of the method was proved using stress conditions and evaluated using a PDA detector. The data validation showed that the method is specific, fast, accurate, and reproducible for the determination of Fe-bis-gly in dosage form. The response was linear over a range of 1.0–2.6 μg mL−1 (r = 0.9999). The accuracy of the method ranged from 98.02 to 102.75%. The RSD values for intra- and inter-day precision studies were below 1.3 and 1.1%, respectively. There was no interference of the excipients on the determination of the active pharmaceutical ingredient.

Keywords

Column liquid chromatographyIon-pairing liquid chromatographyValidationFerrous bisglycinate4-(2-Pyridylazo) resorcinol

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2009

Authors and Affiliations

  • Danieli Cátia Ceni
    • 1
  • Laura Alegria Martins
    • 1
  • Andréa Garcia Pereira
    • 1
  • Pedro Eduardo Fröehlich
    • 1
  • Ana Maria Bergold
    • 1
  1. 1.Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de FarmáciaUniversidade Federal do Rio Grande do Sul (UFRGS)Porto AlegreBrazil