Chromatographia

, Volume 68, Issue 7, pp 623–627

Simultaneous Determination of Nicotine and Its Nine Metabolites in Human Urine by LC–MS–MS

  • Zhong Fan
  • Fuwei Xie
  • Qiaoling Xia
  • Sheng Wang
  • Li Ding
  • Huimin Liu
Full Short Communication

DOI: 10.1365/s10337-008-0729-9

Cite this article as:
Fan, Z., Xie, F., Xia, Q. et al. Chroma (2008) 68: 623. doi:10.1365/s10337-008-0729-9

Abstract

A method based on liquid chromatography tandem mass spectrometry was developed for the direct determination of nicotine, cotinine, trans-3′-hydroxycotinine, their corresponding glucuronide conjugates as well as nornicotine, norcotinine, cotinine-N-oxide and nicotine-N-oxide in the urine of smokers. The assay only involves centrifugation and filtration of diluted urine. The analysis was performed on a C18 reversed-phase column using a gradient of 10 mM ammonium acetate, pH 6.8, and methanol as mobile phase at a flow rate of 1 mL min−1. Nicotine-methyl-d3, Cotinine-methyl-d3 and trans-3′-hydroxycotinine-methyl-d3 were used as internal standards. Precisions (RSD) for all the analytes at three levels were between 2.1 and 17.0%. Recoveries for nicotine and nine nicotine metabolites ranged from 78.4 to 115.6%. The described method was suitable for determining the nicotine dose in large-scale human biomonitoring studies.

Keywords

Column liquid chromatography–tandem mass spectrometryNicotine metabolites in human urine

Copyright information

© Vieweg+Teubner | GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Zhong Fan
    • 1
  • Fuwei Xie
    • 1
  • Qiaoling Xia
    • 1
  • Sheng Wang
    • 1
  • Li Ding
    • 1
  • Huimin Liu
    • 1
  1. 1.Chemistry DepartmentZhengzhou Tobacco Research Institute of CNTCZhengzhouPeople’s Republic of China