Abstract
A simple, rapid and precise reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of eugenol from the extract of dried powder of Cinnamomum tamala leaves and its polyherbal formulation. Chromatographic analysis was carried out on Zorbax C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of mixture of water, acetonitrile and methanol in the volume ratio of 50:40:10, at a flow rate of 1.0 mL min-1. Quantitation was performed using a UV-visible detector at 210 nm. Good linearity was obtained over the ranges of 0.20–3.0 μg mL-1 for eugenol.
References
Showkat RM, Mohammed A, Kapoor R (2004) Flavour Fragrance J 19:112–114
Kirtikar and Basu (1981) Indian Medicinal Plants. Second Edition, Published by Lalit- Mohan Basu, India, 3:2146–2147
Kapoor LD (2000) CRC Handbook of Ayurvedic Medicinal Plants CRC Press, Inc. Boca Raton, Florida, pp 117
Kar A, Choudhary BK, Bandyopadhyay NG (2003) J Ethnopharmacolgy 84:105–108
Chang Shang-Tzen, Cheng Sen-Sung (2002) J Agric and Food Chem 50(6):1389–1392
Smith RL, Adams TB, Doull J, Feron VJ, Goodman JI, Marnett LJ, Portoghese PS, Waddell WJ, Wagner BM, Rogers AE, Caldwell J, Sipes IG (2002) Food Chem Toxicol 40:851–870
Saino F, Ghizzoni C, Gionfriddo F, Colombo E, Servillo L, Castaldo D (2003) Food Chem 81:469–475
Fischer IU, Dengler HJ (1990) J Chromatogr 525:369–377
Kermasha S, Goetghebeur M, Dumont J (1994) Lebensm-Wiss Technol 27(6):578–582
Yuwono M, Siswandono, Hafid AF, Poernomo AT, Agil M, Indrayanto G, Ebel S, (2002) Analytical Profiles and Drug Substances and Excipients. Academic Press, Elsevier Sceince (USA) 29:149–177
Snyder LR, Kirland JJ, Glajch JL (1997) Practical HPLC method development, Second Edition, John Wiley and Sons, Inc., U.S.A
Validation of Analytical Procedures (1996):Methodology, ICH Harmonised Tripartite guidelines, Adoption on 6 November
Eksteen KR, Schoenmakers P (1998) Chromatography Science Series, Handbook of HPLC, Vol 78
Szepesi G (1992) How to used reversed phase HPLC? VCH Publishers, Inc. USA
Text on Validation of Analytical Procedures, (1994), ICH Harmonised Tripartite guidelines, Adoption on 27 October
Myint S, Daud WRW, Mohamad BA, Kadhum HAA (1996) J Chromatogr B 679:193–195
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Revised: 15 October 2004 and 31 January 2005
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Dighe, V.V., Gursale, A.A., Sane, R.T. et al. Quantitative Determination of Eugenol from Cinnamomum tamala Nees and Eberm. Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography. Chroma 61, 443–446 (2005). https://doi.org/10.1365/s10337-005-0527-6
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DOI: https://doi.org/10.1365/s10337-005-0527-6