, Volume 61, Issue 9, pp 443-446

First online:

Quantitative Determination of Eugenol from Cinnamomum tamala Nees and Eberm. Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography

  • V. V. DigheAffiliated withDepartment of Chemistry Email author 
  • , A. A. GursaleAffiliated withTherapeutic Drug Monitoring Laboratory
  • , R. T. SaneAffiliated withDepartment of ChemistryTherapeutic Drug Monitoring Laboratory
  • , S. MenonAffiliated withTherapeutic Drug Monitoring Laboratory
  • , P. H. PatelAffiliated withTherapeutic Drug Monitoring Laboratory

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A simple, rapid and precise reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of eugenol from the extract of dried powder of Cinnamomum tamala leaves and its polyherbal formulation. Chromatographic analysis was carried out on Zorbax C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of mixture of water, acetonitrile and methanol in the volume ratio of 50:40:10, at a flow rate of 1.0 mL min-1. Quantitation was performed using a UV-visible detector at 210 nm. Good linearity was obtained over the ranges of 0.20–3.0 μg mL-1 for eugenol.


Column liquid chromatography HPLC Eugenol Cinnamomum tamala Nees Eberm leaf powder