In Vitro Cellular & Developmental Biology - Animal

, Volume 41, Issue 8, pp 278–283

Multicolor karyotype analyses of mouse embryonic stem cells

Authors

  • Jianli Guo
    • Institute of Human GeneticsUniversity of Heidelberg
  • Anna Jauch
    • Institute of Human GeneticsUniversity of Heidelberg
  • Heidi Holtgreve-Grez
    • Institute of Human GeneticsUniversity of Heidelberg
  • Brigitte Schoell
    • Institute of Human GeneticsUniversity of Heidelberg
  • Dorothee Erz
    • Institute of Human GeneticsUniversity of Heidelberg
  • Martina Schrank
    • Institute of Human GeneticsUniversity of Heidelberg
    • Institute of Human GeneticsUniversity of Heidelberg
Articles Cell Growth/Differentiation/Apoptosis

DOI: 10.1290/990771.1

Cite this article as:
Guo, J., Jauch, A., Holtgreve-Grez, H. et al. In Vitro Cell.Dev.Biol.-Animal (2005) 41: 278. doi:10.1290/990771.1

Summary

The manipulation of embryonic stem (ES) cells to introduce directional genetic changes into the genome of mice has become an important tool in biomedical research. Monitoring of cell morphology before and after DNA manipulation and special culture conditions are a prerequisite to preserve the pluripotent properties of ES cells and thus their ability to generate chimera and effective germline transmission (GLT). It has been reported that prolonged cell culturing may affect the diploid chromosomal composition of cells and therefore the percentage of chimerism and GLT. Herein, we report multicolor-fluorescence in situ hybridization (M-FISH) analysis of four different ES cell lines/clones. Although the morphology of all four ES cell lines/clones appeared normal and all four expressed the early markers Oct-3/4 and Nanog, two cell lines presented consistent numerical and structural chromosome aberrations. We demonstrate that M-FISH is a sensitive and accurate method for a comprehensive karyotype analysis of ES cells and may minimize time, costs, and disappointment due to inadequate ES cell sources.

Key words

multicolor karyotype analyses M-FISH ES cells LIF feeder cells

Copyright information

© Society for In Vitro Biology 2005