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Expression of secreted frizzled-related protein 2 in a primary canine mammary tumor cell line: A candidate tumor marker for mammary tumor cells

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Summary

Canine mammary tumors (CMTs) have been proposed to be a good animal model for human breast cancer. To provide a basis for the tumorigenic study of CMTs, cell lines were established using a modified cell culture technique. The epithelial morphology and immunostaining with cytokeratin 18 confirmed the epithelial origin of the cells. In an investigation of possible mammary tumorigenesis-related factors, the expression of Wnt signaling-related proteins was detected in cell lines. Secreted frizzled-related protein 2 (SFRP2) was abundantly expressed in CMT cells but not in normal canine mammary gland (MG) cells. Secrted frizzled-related protein 2 was secreted into the culture medium and was associated with the extracellular matrix. In addition, increased expressions of β-catenin and cyclin D1 were observed in cells overexpressing SFRP2. The marked differential expression of SFRP2 reveals that this protein may be a potential candidate marker for CMTs. The CMT cell line established in this study provides a useful tool and experimental model for understanding both the tumorigenesis of CMTs and the role of Wnt signaling in cancers.

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Authors and Affiliations

  1. Graduate Institute of Veterinary Medicine, National Taiwan University, 106, Taipei, Taiwan, Republic of Chinca

    Jia-Lin Lee & Ling-Ling Chueh

  2. Institute of Biological Chemistry, Academia Sinica, 115, Taipei, Taiwan, Republic of China

    Ching-Jin Chang & Chung-Tien Lin

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  1. Jia-Lin Lee
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  2. Ching-Jin Chang
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Correspondence to Chung-Tien Lin.

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Lee, JL., Chang, CJ., Chueh, LL. et al. Expression of secreted frizzled-related protein 2 in a primary canine mammary tumor cell line: A candidate tumor marker for mammary tumor cells. In Vitro Cell.Dev.Biol.-Animal 39, 221–227 (2003). https://doi.org/10.1290/1543-706X(2003)039<0221:EOSFPI>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1543-706X(2003)039<0221:EOSFPI>2.0.CO;2

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