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In vitro culture of rat hepatocytes without exogenous matrix

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References

  • Bissel, D. M.; Guzelian, P. S. Microsomal functions and phenotypic change in adult rat hepatocytes in primary monolayer culture. In: Gerschensan, L. E.; Thompson, E. B., ed. International symposium on gene expression and carcinogenesis in culture liver. New York: Academic Press; 1980:119–138.

    Google Scholar 

  • Donato, M. T.; Castell, J.; Gómez-Lechon, M. J. Cytochrome P-450 activities in pure and co-cultured rat hepatocytes. Effects of model inducers. In Vitro Cell. Dev. Biol. 30A(12):825–832; 1994.

    CAS  Google Scholar 

  • Donato, M. T.; Gómez-Lechón, M. J.; Castell, J. Drug metabolizing enzymes in rat hepatocytes co-cultured with cell lines. In Vitro Cell. Dev. Biol. 26:1057–1062; 1990.

    PubMed  CAS  Google Scholar 

  • Dunn, J. C.; Tompkins, R. G.; Yarmush, M. L. Long term in vitro function of adult hepatocytes in a collagen sandwich configuration. Biotechnol. Prog. 7:237–245; 1991.

    Article  PubMed  CAS  Google Scholar 

  • Enat, R.; Jefferson, D. M.; Ruiz-Opazo, N.; Gatmaitan, Z.; Leinwand, L. A.; Reid, L. M. Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix. Proc. Natl. Acad. Sci. USA 81:1411–1415; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Ferriola, P. C.; Robertson, A. T.; Rusnak, D. W., et al. Epidermal growth factor dependence and TGFα autocrine growth regulation in primary rat tracheal epithelial cells. J. Cell Physiol. 152(2):302–309; 1992.

    Article  PubMed  CAS  Google Scholar 

  • Guengerich, F. P. Isolation and purification of cytochrome P-450, and the existence of multiple forms. Pharmacol. Ther. 6:99–121; 1979.

    Article  PubMed  CAS  Google Scholar 

  • Guguen-Guilleuzo, C.; Clement, B.; Baffet, G.; Beaumont, C.; Morel-Chany, E.; Glaise, D.; Guillouzo, A. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell line. Exp. Cell Res. 143:47–54; 1983.

    Article  Google Scholar 

  • Hammond, S. L.; Ham, R. G.; Stampler, M. R.; Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract. Proc. Natl. Acad. Sci. USA 81:5435–5439; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Hu, M. Y.; Cipolle, M.; Sielaff, T., et al. Effects of hepatocyte growth factor on viability and biotransformation functions of hepatocytes in gel entrapped and monolayer culture. Crit. Care Med. 23(7):1237–1242; 1995.

    Article  PubMed  CAS  Google Scholar 

  • Ijima, H.; Nakazawa, K.; Mizumoto, H.; Matsushita, T.; Funatsu, K. Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver. J. Biomater. Sci. Polym. Ed. 9(7):765–778; 1998.

    PubMed  CAS  Google Scholar 

  • Johansson, S.; Hook, M. Substrate adhesion of rat hepatocytes. On the mechanism of attachment of fibronectin. J. Cell Biol. 98:810–817; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Karikusa, F. The restoration of the functions of serially passaged calf hepatocytes by spheroid formation. In Vitro Cell. Dev. Biol. 32:30–37; 1996.

    CAS  Google Scholar 

  • Koide, N.; Sakaguchi, K.; Koide, Y., et al. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp. Cell Res. 186:227–235; 1990.

    Article  PubMed  CAS  Google Scholar 

  • Lazar, A.; Mann, H. J.; Remmel, R. P.; Shatford, R. A.; Cerra, F. B.; Hu W. S. Extended liver-specific functions of porcine hepatocyte spheroids entrapped in collagen gel. In Vitro Cell. Dev. Biol. 31:340–346; 1995a.

    CAS  Google Scholar 

  • Lazar, A.; Peshwa, M. V.; Wu, F.; Chi, C. M.; Cerra, F. B.; Hu, W. S. Formation of porcine hepatocyte spheroids for use in a bioartificial liver. Cell Transplantation 4(3):259–268; 1995b.

    Article  PubMed  CAS  Google Scholar 

  • Lilja, H.; Blanc, P.; Demetriou, A. A.; Rozga, J. Response of cultured fetal and adult rat hepatocytes to growth factors and cyclosporine. Cell Transplantation 7(3):257–66; 1998.

    Article  PubMed  CAS  Google Scholar 

  • Lobo, J. O.; Samrock, R. L.; Jayme, D. W.; Price, P. J. Sustained inducibility of cytochrome P-450 activity in rat hepatocytes cultured in a serum free medium. In: Kaminogawa, S.; Amentani, A.; Hachimura, S., eds. Animal cell technology: basic & applied aspects. Volume 5, Dordrecht, Holland: Kluwer Academic Publishers; 1993:195–201.

    Google Scholar 

  • Michalopoulos, G.; Pitot, H. C. Primary culture of parenchymal cells on collagen membranes. Exp. Cell Res. 94:70–78; 1975.

    Article  PubMed  CAS  Google Scholar 

  • Michalopoulos, G. K.; Bowen, W. C.; Zajac, V. F., et al. Morphogenetic events in mixed cultures of rat hepatocytes and non-parenchymal cells maintained in biological matrices in the presence of hepatocyte growth factor and epidermal growth factor. Hepatology 29(1):90–100; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Mitaka, T.; Kojima, T.; Mizuguchi, T.; Mochizuki, Y. Subculture of proliferating adult rat hepatocytes in medium supplemented with nicotinamide and EGF. In Vitro Cell. Dev. Biol. 32:469–477; 1996.

    CAS  Google Scholar 

  • Mitaka, T.; Norioka, K.; Nakamura, T. Effects of mitogen and co-mitogens on the formation of small-cell colonies in primary cultures of rat hepatocytes. J. Cell. Physiol. 157:461–468; 1993.

    Article  PubMed  CAS  Google Scholar 

  • Mitaka, T.; Sato, F.; Mizuguchi, T.; Yokono, T.; Mochizuki, Y. Reconstruction of hepatic organoid by rat small hepatocytes and hepatic nonparenchymal cells. Hepatology 29(1):111–125; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Naik, S.; Santangini, H.; Gann, K.; Jauregui, H. Influence of different substrates in detoxification activity of adult rat hepatocytes in long-term culture: implications for transplantation. Cell Transplantation 1:61–69; 1992.

    PubMed  CAS  Google Scholar 

  • Nishikawa, Y.; Tokusashi, Y.; Kadohama, T. Hepatocytic cells from bile ductlike structures within a three-dimensional collagen gel matrix. Exp. Cell Res. 223:357–371; 1996.

    Article  PubMed  CAS  Google Scholar 

  • Peshwa, M. V.; Wu, F. J.; Sharp, H. L.; Cerra, F. B.; Hu, W. S.. Mechanistic of formation and ultrastructural evaluation of hepatocyte spheroids. In Vitro Cell. Dev. Biol. 32:197–203; 1996.

    CAS  Google Scholar 

  • Pittelkow, M. R.; Shipley, G. D. Serum free culture of normal human melanocytes: growth kinetics and growth factors requirements. J. Cell Physiol. 140(3):565–576; 1989.

    Article  PubMed  CAS  Google Scholar 

  • Rana, B.; Mischoulon, D.; Xie, Y., et al. Cell-extracellular matrix interactions can regulate the switch between growth and differentiation in rat hepatocytes: reciprocal expression of C/EBPα and immediate early growth response transcription factors. Mol. Cell. Biol. 14:5858–5869; 1994.

    PubMed  CAS  Google Scholar 

  • Reid, L. M.; Jefferson, D. M. Culturing hepatocytes and other differentiated cells. Hepatology 4(3):548–559; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Runge, D. M.; Runge, D.; Dorko, K., et al. Epidermal growth factor- and hepatocyte growth factor-receptor activity in serum-free cultures of human hepatocytes. J. Hepatol. 30:265–274; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Schuetz, E. G.; Li, D.; Omiecinski, C. J.; Muller-Eberhard, U.; Kleinman, H. K.; Elswick, B.; Guzelian, P. S. Regulation of gene expression in adult rat hepatocytes cultured on a basement membrane matrix. J. Cell Physiol. 134:309–323; 1988.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Unit of Experimental Medicine, Hospital Italiano de Buenos Aires, Gascón 450, 1181, Buenos Aires, Argentina

    Mariana Barbich, Alicia Lorenti, Alejandra Hidalgo, Catalina B. de Di Risio, Sung Ho Hyon & Pablo Argibay

  2. Clinical Laboratory, Hospital Italiano de Buenos Aires, Gascón 450, 1181, Buenos Aires, Argentina

    Patricia Sorroche

  3. Pathology Service, Hospital Italiano de Buenos Aires, Gascón 450, 1181, Buenos Aires, Argentina

    Esteban Mocetti

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  1. Mariana Barbich
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  2. Alicia Lorenti
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  3. Patricia Sorroche
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  4. Esteban Mocetti
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  5. Alejandra Hidalgo
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  6. Catalina B. de Di Risio
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  7. Sung Ho Hyon
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  8. Pablo Argibay
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Correspondence to Pablo Argibay.

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Barbich, M., Lorenti, A., Sorroche, P. et al. In vitro culture of rat hepatocytes without exogenous matrix. In Vitro Cell.Dev.Biol.-Animal 36, 405–409 (2000). https://doi.org/10.1290/1071-2690(2000)036<0405:IVCORH>2.0.CO;2

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  • DOI: https://doi.org/10.1290/1071-2690(2000)036<0405:IVCORH>2.0.CO;2

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