Summary
Cell growth in ovarian primary culture of the kuruma shrimp, Marsupenaeus japonicus, was examined under various culture conditions. The best growth of ovarian cells was obtained in a culture system consisting of double strength of Leibowitz-15 medium supplemented with 10% fetal bovine serum, glucose (1 g/L), proline (0.1 g/L), TC-Yeastolate (1 g/L), and lactalbumin hydrolysate (1 g/L). The cells survived in this medium at 25° C for 45 d. The epithelial-like cells predominated in 10-d-old cultures, covering >80% of the surface area on the bottom of flask. Cells in mitosis were often observed. Cell proliferation was monitored by incorporation of 5-bromo-2′-deoxyuridine (BrdU), an analog of thymidine. 5-Bromo-2′-deoxyuridine-associated cells accounted for 11.5 and 35.0% of cell populations at 2 and 24 h, respectively, after BrdU treatment. Our results provide an improved culture technique for ovarian tissue of the kuruma shrimp.
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Maeda, M., Mizuki, E., Itami, T. et al. Ovarian primary tissue culture of the kuruma shrimp Marsupenaeus japonicus . In Vitro Cell.Dev.Biol.-Animal 39, 208–212 (2003). https://doi.org/10.1290/1543-706X(2003)039<0208:OPTCOT>2.0.CO;2
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DOI: https://doi.org/10.1290/1543-706X(2003)039<0208:OPTCOT>2.0.CO;2