Human myocardial cell lines generated with SV40 temperature-sensitive mutant TSA58
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Conditionally transformed human myocardial cell lines would be a valuable resource for studying human cardiac cell biology. We generated clonal human fetal cardiocyte cell lines by transfection of fetal ventricular cardiac cell clones with a plasmid containing a replication-defective mutant of the temperature-sensitive SV40 strain tsA58. Multiple resulting cell lines showed similar features, namely: (1) T antigen (TAg) expression at both permissive (34°C) and restrictive (40.5°C) temperatures; (2) extended growth capacity in comparison with parental wild type, when grown at the permissive temperature; (3) both temperature-dependent and serum-responsive growth, and; (4) an incompletely differentiated fetal phenotype which was similar at both permissive and restrictive temperatures and in the presence and absence of serum. The transformed myocyte phenotype was demonstrated using immunocytochemistry, Western and Northern blotting, and reverse transcription-polymerase chain reaction (RT-PCR). Cell lines expressed skeletal α-actin, atrial natriuretic peptide (ANP), and keratins, but no sarcomeric myosin heavy chain or desmin. Immunoreactive sarcomeric actin was expressed predominantly as a truncated protein of approximately 38 kD. The phenotype of the transformed cells differs from that of the wild-type parental cells as well as from those reported by others who have used TAg to immortalize rodent or human ventricular myocytes. Our cell lines should provide a useful tool for study of the molecular mechanisms regulating growth and differentiation in human cardiac muscle cells.
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- Human myocardial cell lines generated with SV40 temperature-sensitive mutant TSA58
In Vitro Cellular & Developmental Biology - Animal
Volume 42, Issue 10 , pp 324-331
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- fetal heart
- myocardial differentiation
- viral transfection
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- Author Affiliations
- 1. Department of Pathology and Laboratory Medicine, Temple University School of Medicine, Philadelphia, USA
- 3. Department of Surgery, University of Pennsylvania School of Medicine, Pennsylvarnia, USA
- 4. Department of Physiology, Temple University School of Medicine, Philadelphia, USA
- 5. Department of Surgery, Division of Cardiothoracic Surgery, Temple University School of Medicine, Philadelphia, USA