In Vitro Cellular & Developmental Biology - Animal

, Volume 41, Issue 7, pp 232–238

In vitro differentiation and mineralization of human dental pulp cells induced by dentin extract

Authors

  • Jun Liu
    • Department of Cariology, Restorative Sciences, and Endodontics, School of DentistryUniversity of Michigan
  • Taocong Jin
    • Department of Cariology, Restorative Sciences, and Endodontics, School of DentistryUniversity of Michigan
  • Helena H. Ritchie
    • Department of Cariology, Restorative Sciences, and Endodontics, School of DentistryUniversity of Michigan
  • Anthony J. Smith
    • Department of Oral Biology, School of DentistryUniversity of Birmingham
    • Department of Cariology, Restorative Sciences, and Endodontics, School of DentistryUniversity of Michigan
Articles Cell Growth/Differentiation/Apoptosis

DOI: 10.1290/0502014.1

Cite this article as:
Liu, J., Jin, T., Ritchie, H.H. et al. In Vitro Cell.Dev.Biol.-Animal (2005) 41: 232. doi:10.1290/0502014.1

Summary

In this study, the progenitor cells isolated from the human dental pulp were used to study the effects of ethylenediaminetetraacetic acid-soluble dentin extract (DE) on their differentiation and mineralization to better understand tissue injury and repair in the tooth. Mineralization of the matrix was increasingly evident at 14, 21, and 28 d after treatment with a mineralization supplement (MS) (ascorbic acid [AA], β-glycerophosphate [β-GP]) and MS+DE. Real-time polymerase chain reaction results showed type I collagen upregulation after the addition of MS+DE at 7 d. Alkaline phosphatase was downregulated after the mineralization became obvious at 14 d. Bone sialoprotein was shown to be upregulated in the mineralized cell groups at all time points and dentin sialophosphoprotein after 7 d. Core binding factor a 1 was upregulated by the treatment of MS and DE at 7, 14, and 21 d. These results indicated that the MS of AA, β-GP, and DE synergistically induced cell differentiation of pulp progenitor cells into odontoblast-like cells and induced in vitro mineralization.

Key words

progenitor celldentin sialophosphoproteinbone sialoproteincore binding factor a 1mineralization

Copyright information

© Society for In Vitro Biology 2005