Articles Biotechnology

In Vitro Cellular & Developmental Biology - Animal

, Volume 41, Issue 7, pp 188-196

A novel composite scaffold for cardiac tissue engineering

  • Hyoungshin ParkAffiliated withHarvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
  • , Milica RadisicAffiliated withHarvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology
  • , Jeong Ok LimAffiliated withMedical Research Institute, Kyungpook National UniversityInstitute for Regenerative Medicine, Wake Forest School of Medicine
  • , Bong Hyun ChangAffiliated withDepartment of Thoracic and cardiovascular Surgery, Kyungpook National University, School of Medicine
  • , Gordana Vunjak-NovakovicAffiliated withHarvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology Email author 

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Summary

One approach to the engineering of functional cardiac tissue for basic studies and potential clinical use involves bioreactor cultivation of dissociated cells on a biomaterial scaffold. Our objective was to develop a scaffold that is (1) highly porous with large intereconnected pores (to facilitate mass transport), (2) hydrophilic (to enhance cell attachment), (3) structurally stable (to withstand the shearing forces during bioreactor cultivation), (4) degradable (to provide ultimate biocompatibility of the tissue graft), and (5) elastic (to enable transmission of contractile forces). The scaffold of choice was made as a composite of poly(Dl-lactide-co-caprolactone), poly(Dl-lactide-co-glycolide) (PLGA), and type I collagen, with open interconnected pores and the average void volume of 80±5%. Neonatal rat heart cells suspended in Matrigel were seeded into the scaffold at a physiologically high density (1.35×108 cells/cm3) and cultivated for 8 d in cartridges perfused with culture medium or in orbitally mixed dishes (25 rpm); collagen sponge (Ultrafoam⋆m) and PLGA sponge served as controls. Construct cellularity, presence of cardiac markers, and contractile properties were markedly improved in composite scaffolds as compared with both controls.

Key words

cardiac myocyte collagen caprolactone PLGA biocompatibility perfusion