Articles Cell Growth/Differentiation/Apoptosis

In Vitro Cellular & Developmental Biology - Animal

, Volume 41, Issue 3, pp 104-110

First online:

Modulation of activin a—Induced differentiation in vitro by vascular endothelial growth factor in Xenopus presumptive ectodermal cells

  • Shiro YoshidaAffiliated withDepartment of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo
  • , Miho FurueAffiliated withDepartment of Biochemistry and Molecular Biology, Kanagawa Dental College
  • , Kentaro NagamineAffiliated withEiken Chemical Co. Ltd.
  • , Takanori AbeAffiliated withDepartment of Biological Science, Graduate School of Science, The University of Tokyo
  • , Yasuto FukuiAffiliated withDepartment of Molecular Oral Medicine and Maxillofacial Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • , Yasufumi MyoishiAffiliated withDepartment of Molecular Oral Medicine and Maxillofacial Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • , Tomoyuki FujiiAffiliated withDepartment of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo
  • , Tetsuji OkamotoAffiliated withDepartment of Molecular Oral Medicine and Maxillofacial Surgery, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University
  • , Yuji TaketaniAffiliated withDepartment of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo
    • , Makoto AsashimaAffiliated withInternational Cooperative Research Project (ICORP)-Japan Science and Technology Agency, The University of TokyoDepartment of Life Sciences (Biology), Graduate School of Arts and Sciences, The University of Tokyo

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Summary

We have previously demonstrated that activin A at low concentrations induced ventral mesoderm including blood-like cells from Xenopus animal caps and that beating heart could be also induced from animal caps treated with 100 ng/ml activin A, suggesting that activin A might be involved in cardiac vasculogenesis. A vascular endothelial growth factor (VEGF) is a powerful mitogen for endothelial cells and is an inducer and regulator of angiogenesis. However, VEGF function in Xenopus development is not clearly identified. In this study, we determined the effect of VEGF on activin A—induced differentiation of animal cap. The VEGF induced duct-like structure composed of Flk-1-positive cells together with the induction of nonvascular tissues, such as neural tissues. This histological result was coincident with our reverse transcriptase-polymerase chain reaction analysis that VEGF together with activin A promoted the expression of Xenopus N-CAM and Xenopus brachyury. This study suggests that VEGF has additional biological activities besides angiogenesis, and arises a different function that VEGF induces stroma cell migration or recruitment that are required for blood vessel formation. This differentiation system will aid in the understanding of angiogenesis during early development.

Key words

activin VEGF blood vessel endothelial cell animal cap