Effects of anaerobic exercise and aerobic exercise on biomarkers of oxidative stress
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In addition to having health-promoting effects, exercise is considered to induce oxidative stress. To clarify whether increased oxygen consumption during exercise induces oxidative stress, we investigated the effects of aerobic exercise and anaerobic exercise on a series of oxidative damage markers.
One group of subjects performed aerobic exercise and another group performed anaerobic exercise with similar workloads, but with different levels of oxygen consumption. Blood and urine samples were collected before, immediately after, and 3, 9, and 24 h after exercise. Serum uric acid (UA) and creatine phosphokinase were evaluated. As markers of oxidative damage to lipids, proteins and DNA, we evaluated serum 4-hydroxy-2-nonenal, urinary F2-isoprostanes, serum protein carbonyls, and leukocyte 8-hydroxydeoxyguanosine.
Oxygen consumption was significantly greater during aerobic exercise. Although UA level increased immediately after aerobic exercise and decreased thereafter, UA level did not change after anaerobic exercise. The two types of exercise had significantly different effects on the change in UA level. After anaerobic exercise, the levels of 8-hydroxydeoxyguanosine and 4-hydroxy-2-nonenal significantly increased at 24 h and 3 h, respectively. The levels of creatine phosphokinase and F2-isoprostanes decreased after exercise. The two types of exercise caused no apparent significant differences in the levels of these biomarkers.
The findings suggest that similar workloads of anaerobic exercise and aerobic exercise induce reactive oxygen species (ROS) differently: aerobic exercise seems to initially generate more ROS, whereas anaerobic exercise may induce prolonged ROS generation. Although more oxygen was consumed during aerobic exercise, the generated ROS did not induce significant oxidative damage. Oxygen consumption per se may not be the major cause of exercise-induced oxidative damage.
- Gutteridge JM, Halliwell B. Free radicals and antioxidants in the year 2000. A historical look to the future. Ann NY Acad Sci. 2000;899:136–147. CrossRef
- Halliwell B. Free radicals and antioxidants: a personal view. Nutr Rev. 1994;52:253–265.
- Paffenberger RS, Jr., Hyde RT, Wing AL, Lee IM, Jung DL, Kampert JB. The association of changes in physical-activity level and other lifestyle characteristics with mortality among men. N Engl J Med. 1993;328:538–545. CrossRef
- Shephard RJ, Shek PN. Associations between physical activity and susceptibility to cancer: possible mechanisms. Sports Med. 1998;26:293–315. CrossRef
- Jenkins RR. Exercise and oxidative stress methodology: a critique. Am J Clin Nutr. 2000;72:670S-674S.
- Singh VN. A current perspective on nutrition and exercise. J Nutr. 1992;122:760–765.
- Sjodin B, Westing YH, Apple FS. Biochemical mechanisms for oxygen free radical formation during exercise. Sports Med. 1990;10:236–254. CrossRef
- Koyama K, Kaya M, Ishigaki T, Tsujita J, Hori S, Seino T, et al. Role of xanthine oxidase in delayed lipid peroxidation in rat liver induced by acute exhausting exercise. Eur J Appl Physiol Occup Physiol. 1999;80:28–33. CrossRef
- Hessel E, Haberland A, Muller M, Lerche D, Schimke I. Oxygen radical generation of neutrophils: a reason for oxidative stress during marathon running? Clin Chim Acta 2000; 298:145–156. CrossRef
- Ji LL. Oxidative stress during exercise: implication of antioxidant nutrients. Free Radic Biol Med. 1995;18:1079–1086. CrossRef
- Jackson M. Exercise and oxygen radical production by muscle. In: Sen C, Packer L, Hanninen O editors. Handbook of Oxidants and Antioxidants in Exercise. Amsterdam: Elsevier; 2000, p. 57–68. CrossRef
- Zouhal H, Rannou F, Gratas-Delamarche A, Monnier M, Bentue-Ferrer D, Delamarche P. Adrenal medulla responsiveness to the sympathetic nervous activity in sprinters and untrained subjects during a supramaximal exercise. Int J Sports Med. 1998;19:172–176. CrossRef
- Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL. Generation of reactive oxygen species after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc. 2000;32:1576–1581. CrossRef
- Ashton T, Young IS, Peters JR, Jones E, Jackson SK, Davies B, et al. Electron spin resonance spectroscopy, exercise, and oxidative stress: an ascorbic acid intervention study. J Appl Physiol. 1999;87:2032–2036.
- Child RB, Wilkinson DM, Fallowfield JL, Donnelly AE. Elevated serum antioxidant capacity and plasma malondialdehyde concentration in response to a simulated half-marathon run. Med Sci Sports Exerc. 1998;30:1603–1607. CrossRef
- Okamura K, Doi T, Hamada K, Sakurai M, Yoshioka Y, Mitsuzono R, et al. Effect of repeated exercise on urinary 8-hydroxy-deoxyguanosine excretion in humans. Free Radic Res. 1997;26:507–514. CrossRef
- Duthie GG, Robertson JD, Maughan RJ, Morrice PC. Blood antioxidant status and erythrocyte lipid peroxidation following distance running. Arch Biochem Biophys. 1990;282:78–83. CrossRef
- Margaritis I, Tessier F, Richard MJ, Marconnet P. No evidence of oxidative stress after a triathlon race in highly trained competitors. Int J Sports Med. 1997;18:186–190. CrossRef
- Bloomer RJ, Goldfarb AH, Wideman L, McKenzie MJ, Consitt LA. Effects of acute aerobic and anaerobic exercise on blood markers of oxidative stress. J Strength Cond Res. 2005;19:276–285. CrossRef
- Finaud J, Scislowski V, Lac G, Durand D, Vidalin H, Robert A, et al. Antioxidant status and oxidative stress in professional rugby players: evolution throughout a season. Int J Sports Med. 2006;27:87–93. CrossRef
- Steinberg JG, Delliaux S, Jammes Y. Reliability of different blood indices to explore the oxidative stress in response to maximal cycling and static exercises. Clin Physiol Funct. Imaging 2006;26:106–112. CrossRef
- Garcia A, Niubo J, Benitez MA, Viqueira M, Perez JL, Comparison of two leukocyte extraction methods for cytomegalovirus antigenemia assay. J Clin Microbiol. 1996;34:182–184.
- Nakajima M, Takeuchi T, Morimoto K. Determination of 8-hydroxydeoxyguanosine in human cells under oxygen-free conditions. Carcinogenesis. 1996;17:787–791. CrossRef
- Takeuchi T, Nakajima M, Ohta Y, Mure K, Takeshita T, Morimoto K. Evaluation of 8-hydroxydeoxyguanosine, a typical oxidative DNA damage, in human leukocytes. Carcinogenesis. 1994;15:1519–1523. CrossRef
- Toyokuni S, Yamada S, Kashima M, Ihara Y, Yamada Y, Tanaka T, et al. Serum 4-hydroxy-2-nonenal-modified albumin is elevated in patients with type 2 diabetes mellitus. Antioxid Redox Signal. 2000;2:681–685. CrossRef
- Uchida K, Stadtman ER. Modification of histidine residues in proteins by reaction with 4-hydroxynonenal. Proc Natl Acad Sci USA. 1992;89:4544–4548. CrossRef
- Shi M, Xu B, Wang X, Aoyama K, Michie SA, Takeuchi T. Oxidative damages in chronic inflammation of a mouse autoimmune disease model. Immunol Lett. 2004;95:233–236. CrossRef
- Song TM. Effect of anaerobic exercise on serum enzymes of young athletes. J Sports Med Phys Fitness. 1990;30:138–141.
- Gutenbrunner C. Circadian variations of the serum creatine kinase level—a masking effect? Chronobiol Int. 2000;17:583–590. CrossRef
- Byrne NM, Hills AP, Hunter GR, Winsier RL, Schutz Y, Metabolic equivalent: one size does not fit all. J Appl Physiol. 2005;99:1112–1119. CrossRef
- Devgun MS, Dhillon HS. Importance of diurnal variations on clinical value and interpretation of serum urate measurements. J Clin Pathol. 1992;45:110–113. CrossRef
- Green HJ, Fraser IG. Differential effects of exercise intensity on serum uric acid concentration. Med Sci Sports Exerc. 1988;20:55–59. CrossRef
- Cuevas MJ, Almar M, Garcia-Glez JC, Garcia-Lopez D, De Paz JA, Alvear-Ordenes I, et al. Changes in oxidative stress markers and NF-kappaB activation induced by sprint exercise. Free Radic Res. 2005;39:431–439. CrossRef
- Galassetti PR, Nemet D, Pescatello A, Rose-Gottron C, Larson J, Cooper DM. Exercise, caloric restriction, and systemic oxidative stress. J Investig. Med. 2006;54:67–75. CrossRef
- Inoue T, Mu Z, Sumikawa K, Adachi K, Okochi T. Effect of physical exercise on the content of 8-hydroxydeoxyguanosine in nuclear DNA prepared from human lymphocytes. Jpn J Cancer Res. 1993;84:720–725.
- Chevion S, Moran DS, Heled Y, Shani Y, Regev G, Abbou B, et al. Plasma antioxidant status and cell injury after severe physical exercise. Proc Natl Acad Sci USA. 2003;100:5119–5123. CrossRef
- Effects of anaerobic exercise and aerobic exercise on biomarkers of oxidative stress
Environmental Health and Preventive Medicine
Volume 12, Issue 5 , pp 202-208
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- aerobic exercise
- anaerobic exercise
- oxidative stress
- uric acid
- Author Affiliations
- 1. Department of Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, 890-8544, Kagoshima, Japan
- 2. Division of Antiviral Chemotherapy, Center for Chronic Viral Diseases at Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan
- 3. Department of Physiological Sciences, National Institute of Fitness and Sports, Kanoya, Japan