Promoter analysis by saturation mutagenesis
- Nitin S. Baliga
- … show all 1 hide
Gene expression and regulation are mediated by DNA sequences, in most instances, directly upstream to the coding sequences by recruiting transcription factors, regulators, and a RNA polymerase in a spatially defined fashion. Few nucleotides within a promoter make contact with the bound proteins. The minimal set of nucleotides that can recruit a protein factor is called a cis-acting element. This article addresses a powerful mutagenesis strategy that can be employed to define cis-acting elements at a molecular level. Technical details including primer design, saturation mutagenesis, construction of promoter libraries, phenotypic analysis, data analysis, and interpretation are discussed.
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- Promoter analysis by saturation mutagenesis
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Biological Procedures Online
Volume 3, Issue 1 , pp 64-69
- Cover Date
- Online ISSN
- Additional Links
- promoter regions (genetics)
- polymerase chain reaction
- Nitin S. Baliga (1)
- Author Affiliations
- 1. Institute for systems Biology, 4225 Roosevelt way NE, Suite 200, 98105, Seattle, WA