Endocrine Tumors

Annals of Surgical Oncology

, Volume 20, Issue 3, pp 759-766

BRAF V600E Mutation Analysis in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-time PCR

  • Dongjun JeongAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine
  • , Yujun JeongAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine
  • , Ji Hye ParkAffiliated withDivision of Breast and Endocrine Surgery, Department of Surgery, Soonchunhyang University, Cheonan Hospital
  • , Sun Wook HanAffiliated withDivision of Breast and Endocrine Surgery, Department of Surgery, Soonchunhyang University, Cheonan Hospital
  • , Sung Yong KimAffiliated withDivision of Breast and Endocrine Surgery, Department of Surgery, Soonchunhyang University, Cheonan Hospital
  • , Yeo Joo KimAffiliated withDivision of Endocrinology, Department of Internal Medicine, Soonchunhyang University, Cheonan Hospital
  • , Sang Jin KimAffiliated withDivision of Endocrinology, Department of Internal Medicine, Soonchunhyang University, Cheonan Hospital
  • , Young HwangboAffiliated withDepartment of Preventive Medicine, Soonchunhyang University College of Medicine
  • , Soyoung ParkAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine
    • , Hyun Deuk ChoAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine
    • , Mee Hye OhAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine
    • , Seung Ha YangAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine
    • , Chang Jin KimAffiliated withDepartment of Pathology, Soonchunhyang University College of Medicine Email author 

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ABSTRACT

Background

Activating somatic mutation of the BRAF V600E has been identified as the most common genetic event in papillary thyroid carcinoma (PTC) with a variable frequency (32–87 %) in different series by different methods. The BRAF V600E mutation is associated with various clinicopathological parameters. The mutation is an important factor for the management of the PTC patients. The objective of this study was to detect the BRAF V600E mutation in PTCs by peptide nucleic acid (PNA) clamp real-time PCR and to analyze the results with clinicopathological parameters.

Methods

We performed genetic analysis of BRAF V600E by PNA clamp real-time PCR in 211 PTCs in Korea, stratified by clinicopathological parameters.

Results

The BRAF V600E mutation was detected in 90 % of PTC cases, and it occurred significantly more often in female patients than in male patients (p = 0.001). The clinicopathological parameters of age, tumor size, and disease stage were not associated with the BRAF V600E mutation, while extrathyroid invasion (p = 0.031), lymph nodal metastasis (p = 0.002), and tumor multiplicity (p = 0.020) were.

Conclusions

The prevalence (90 %) of the BRAF V600E mutation in this study is the highest ever reported, confirming the key role of this mutation in PTC tumorigenesis. The BRAF V600E mutation was associated with aggressive clinical behaviors including extrathyroid invasion, lymph nodal metastasis and tumor multifocality. The PNA clamp real-time PCR method for the BRAF V600E mutation detection is sensitive and is applicable in a clinical setting.