Annals of Surgical Oncology

, Volume 16, Issue 9, pp 2638–2644

Comprehensive Analysis of the Clinical Significance of Inducing Pluripotent Stemness-Related Gene Expression in Colorectal Cancer Cells

Authors

  • Yasumitsu Saiki
    • Department of SurgeryTakano Hospital
    • Department of Surgery, Takano HospitalKyushu University
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
  • Shinya Ishimaru
    • Department of Surgery, Takano HospitalKyushu University
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
  • Koshi Mimori
    • Department of Surgery, Takano HospitalKyushu University
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
  • Yasushi Takatsuno
    • Department of Surgery, Takano HospitalKyushu University
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
  • Makoto Nagahara
    • Department of Surgery, Takano HospitalKyushu University
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
  • Hideshi Ishii
    • Department of Surgery, Takano HospitalKyushu University
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
  • Kazutaka Yamada
    • Department of SurgeryTakano Hospital
    • Department of Surgical Oncology, Medical Institute of BioregulationKyushu University
Translational Research and Biomarkers

DOI: 10.1245/s10434-009-0567-5

Cite this article as:
Saiki, Y., Ishimaru, S., Mimori, K. et al. Ann Surg Oncol (2009) 16: 2638. doi:10.1245/s10434-009-0567-5

Abstract

Background

We previously determined that cancer stem-like cells may influence the susceptibility of colorectal cancer (CRC) cells to chemotherapeutic agents. Although Takahashi and Park identified a set of induced pluripotent stem cell (iPS)-related genes required for normal stem cell maintenance, the precise role of iPS-related gene expression in CRC pathogenesis remains to be determined. The purpose of this study was to clarify the clinical relevance of “stemness”-regulating gene expression in CRC cases.

Materials and methods

Cancer cells were excised from tissues of 79 CRC cases by laser microdissection (LMD), and quantitative RT-PCR was used to evaluate expression levels of the iPS-related genes c-MYC, SOX2, OCT3/4, LIN28, KLF4, and NANOG, and to identify any associations between their expression and clinicopathological CRC progression.

Results

We found that LIN28 expression is significantly associated with lymph node metastasis (p = 0.018) and Dukes stage (p = 0.0319). SOX2expression is also correlated with lymph node metastasis. Furthermore, the ten cases with Dukes D disease expressed significantly higher levels of SOX2transcript than the other 69 cases (p = 0.0136). In contrast, KLF4 expression was inversely related to Dukes stage. Expression of c-MYC, OCT3/4, and NANOG did not appear to have clinical relevance in CRC cases.

Conclusion

The present analysis strongly suggests that altered expression of several iPS-related genes plays a role in CRC pathogenesis.

Copyright information

© Society of Surgical Oncology 2009