Annals of Surgical Oncology

, Volume 22, Issue 8, pp 2649–2655

MiR-92a Promotes Cell Metastasis of Colorectal Cancer Through PTEN-Mediated PI3K/AKT Pathway

  • Tao-Wei Ke
  • Po-Li Wei
  • Ken-Tu Yeh
  • William Tzu-Liang Chen
  • Ya-Wen Cheng
Colorectal Cancer

DOI: 10.1245/s10434-014-4305-2

Cite this article as:
Ke, TW., Wei, PL., Yeh, KT. et al. Ann Surg Oncol (2015) 22: 2649. doi:10.1245/s10434-014-4305-2

Abstract

Background

MicroRNAs regulate gene expression at the posttranscriptional level and play important roles in tumor development, progression, and metastasis. The aim of this study was to investigate the role of microRNA-92a (miR-92a) in metastasis of colorectal cancer (CRC).

Methods

One hundred fifty-eight CRC patients were enrolled. The expression of miR-92a, PTEN, and E-cadherin was analyzed by real-time PCR. Univariate (Kaplan–Meier) analysis was used to analyze primary outcomes included 5-year overall survival and tumor recurrence. CRC cell model studies were used to analyze the miR-92a-involved CRC metastasis.

Results

The expression of miR-92a in tumor tissues was significantly positively correlated with lymph node metastasis in CRC patients (p = 0.012). After adjusting for age, sex, and disease differentiation, this correlation remained significant (p = 0.01). In addition, there was a negative correlation between levels of miR-92a and the PTEN gene (p < 0.0001). No any association of miR-92a and E-cadherin was found (p = 0.128). Patients with high miR-92a/low PTEN had poorer overall survival and disease-free survival rates than those with high miR-92a/high PTEN, low miR-92a/high PTEN, and low miR-92a/low PTEN. The association of levels of miR-92a and PTEN with tumor cell migration in CRC was also confirmed in CRC cell models.

Conclusions

We suggest that miR-92a is involved in lymph node metastasis of CRC patients through PTEN-regulated PI3K/AKT signaling pathway.

Supplementary material

10434_2014_4305_MOESM1_ESM.docx (180 kb)
Supplementary material 1 (DOCX 180 kb)

Copyright information

© Society of Surgical Oncology 2014

Authors and Affiliations

  • Tao-Wei Ke
    • 1
    • 2
  • Po-Li Wei
    • 4
    • 5
    • 6
    • 7
  • Ken-Tu Yeh
    • 3
  • William Tzu-Liang Chen
    • 2
  • Ya-Wen Cheng
    • 7
  1. 1.Institute of MedicineChung-Shan Medical UniversityTaichungTaiwan
  2. 2.Division of Colorectal Surgery, Department of SurgeryChina Medical University HospitalTaichungTaiwan
  3. 3.Department of PathologyChanghua Christian HospitalChanghuaTaiwan
  4. 4.Department of Surgery, College of MedicineTaipei Medical UniversityTaipeiTaiwan
  5. 5.Division of General Surgery, Department of Surgery, Taipei Medical University HospitalTaipei Medical UniversityTaipeiTaiwan
  6. 6.Cancer Center, Taipei Medical University HospitalTaipei Medical UniversityTaipeiTaiwan
  7. 7.Graduate Institute of Cancer Biology and Drug DiscoveryTaipei Medical UniversityTaipeiTaiwan