BRAF V600E Mutation Analysis in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-time PCR
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Activating somatic mutation of the BRAF V600E has been identified as the most common genetic event in papillary thyroid carcinoma (PTC) with a variable frequency (32–87 %) in different series by different methods. The BRAF V600E mutation is associated with various clinicopathological parameters. The mutation is an important factor for the management of the PTC patients. The objective of this study was to detect the BRAF V600E mutation in PTCs by peptide nucleic acid (PNA) clamp real-time PCR and to analyze the results with clinicopathological parameters.
We performed genetic analysis of BRAF V600E by PNA clamp real-time PCR in 211 PTCs in Korea, stratified by clinicopathological parameters.
The BRAF V600E mutation was detected in 90 % of PTC cases, and it occurred significantly more often in female patients than in male patients (p = 0.001). The clinicopathological parameters of age, tumor size, and disease stage were not associated with the BRAF V600E mutation, while extrathyroid invasion (p = 0.031), lymph nodal metastasis (p = 0.002), and tumor multiplicity (p = 0.020) were.
The prevalence (90 %) of the BRAF V600E mutation in this study is the highest ever reported, confirming the key role of this mutation in PTC tumorigenesis. The BRAF V600E mutation was associated with aggressive clinical behaviors including extrathyroid invasion, lymph nodal metastasis and tumor multifocality. The PNA clamp real-time PCR method for the BRAF V600E mutation detection is sensitive and is applicable in a clinical setting.
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- BRAF V600E Mutation Analysis in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-time PCR
Annals of Surgical Oncology
Volume 20, Issue 3 , pp 759-766
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- Dongjun Jeong PhD (1)
- Yujun Jeong MS (1)
- Ji Hye Park MD (2)
- Sun Wook Han MD (2)
- Sung Yong Kim MD, PhD (2)
- Yeo Joo Kim MD, PhD (3)
- Sang Jin Kim MD, PhD (3)
- Young Hwangbo MD, PhD (4)
- Soyoung Park MS (1)
- Hyun Deuk Cho MD, PhD (1)
- Mee Hye Oh MD, PhD (1)
- Seung Ha Yang MD, PhD (1)
- Chang Jin Kim MD, PhD (1)
- Author Affiliations
- 1. Department of Pathology, Soonchunhyang University College of Medicine, Cheonan City, Republic of Korea
- 2. Division of Breast and Endocrine Surgery, Department of Surgery, Soonchunhyang University, Cheonan Hospital, Cheonan City, Republic of Korea
- 3. Division of Endocrinology, Department of Internal Medicine, Soonchunhyang University, Cheonan Hospital, Cheonan City, Republic of Korea
- 4. Department of Preventive Medicine, Soonchunhyang University College of Medicine, Cheonan City, Republic of Korea