Annals of Surgical Oncology

, Volume 20, Issue 5, pp 1737–1744

Biobanking of Fresh-frozen Human Colon Tissues: Impact of Tissue Ex-vivo Ischemia Times and Storage Periods on RNA Quality

  • Wei-Guang Bao
  • Xia Zhang
  • Jian-Gang Zhang
  • Wen-Jun Zhou
  • Tie-Nan Bi
  • Jiao-Chen Wang
  • Wei-Hua Yan
  • Aifen Lin
Translational Research and Biomarkers

DOI: 10.1245/s10434-012-2440-1

Cite this article as:
Bao, WG., Zhang, X., Zhang, JG. et al. Ann Surg Oncol (2013) 20: 1737. doi:10.1245/s10434-012-2440-1

Abstract

Background

Biobanking plays an important role in translational cancer research. The impact of tissue ex-vivo ischemia time and storage period on RNA integrity is not well documented.

Methods

Fresh-frozen colon tissues were collected in Taizhou Hospital of Zhejiang Province in China since 2004. Fifty-one colon cancer tissues with tumor cell content higher than 70 % and matched normal tissues during four storage periods (less than 15 months, 16–20 months, 21–25 months, and 26–40 months) were chosen to detect RNA quality. Fresh colon cancer tissues from 5 patients were cut into pieces and kept at room temperature or on ice for 0.5, 1, 2, and 4 h before snap freezing. RNA integrity was determined by microcapillary electrophoresis by the RNA integrity number (RIN) algorithm.

Results

Sixty-seven percent of normal colon tissues and 94 % of colon cancer specimens yielded RNA with a RIN of ≥7. Matched colon cancer and normal tissues showed significant difference in RNA quality. RNA remained stable in colon cancer tissues kept at room temperature and on ice for up to 4 h, and long-term storage of banked colon specimens did not negatively influence RNA quality (RNA with RIN of ≥7 banked less than 15 months, 83 %; 16–20 months, 78 %; 21–25 months, 77 %; 26–40 months, 90 %).

Conclusions

Frozen colon tissues yield high-quality RNA in approximately 80 % of specimens. Ex-vivo ischemia times and storage periods did not adversely affect RNA quality. This study showed that standard operation protocols and the maintenance of high-quality tissue repositories were the keys to translational medicine research.

Copyright information

© Society of Surgical Oncology 2012

Authors and Affiliations

  • Wei-Guang Bao
    • 1
  • Xia Zhang
    • 1
  • Jian-Gang Zhang
    • 1
  • Wen-Jun Zhou
    • 1
  • Tie-Nan Bi
    • 2
  • Jiao-Chen Wang
    • 3
  • Wei-Hua Yan
    • 4
  • Aifen Lin
    • 1
    • 4
  1. 1.Human Tissue BankTaizhou Hospital of Zhejiang Province, Wenzhou Medical CollegeZhejiangChina
  2. 2.Department of Gastrointestinal SurgeryTaizhou Hospital of Zhejiang Province, Wenzhou Medical CollegeZhejiangChina
  3. 3.Department of PathologyTaizhou Hospital of Zhejiang Province, Wenzhou Medical CollegeZhejiangChina
  4. 4.Medical Research CenterTaizhou Hospital of Zhejiang Province, Wenzhou Medical CollegeZhejiangChina