Three-dimensional Optical Coherence Tomography for Optical Biopsy of Lymph Nodes and Assessment of Metastatic Disease
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Numerous techniques have been developed for localizing lymph nodes before surgical resection and for their histological assessment. Nondestructive high-resolution transcapsule optical imaging of lymph nodes offers the potential for in situ assessment of metastatic involvement, potentially during surgical procedures.
Three-dimensional optical coherence tomography (3-D OCT) was used for imaging and assessing resected popliteal lymph nodes from a preclinical rat metastatic tumor model over a 9-day time-course study after tumor induction. The spectral-domain OCT system utilized a center wavelength of 800 nm, provided axial and transverse resolutions of 3 and 12 μm, respectively, and performed imaging at 10,000 axial scans per second.
OCT is capable of providing high-resolution label-free images of intact lymph node microstructure based on intrinsic optical scattering properties with penetration depths of ~1–2 mm. The results demonstrate that OCT is capable of differentiating normal, reactive, and metastatic lymph nodes based on microstructural changes. The optical scattering and structural changes revealed by OCT from day 3 to day 9 after the injection of tumor cells into the lymphatic system correlate with inflammatory and immunological changes observed in the capsule, precortical regions, follicles, and germination centers found during histopathology.
We report for the first time a longitudinal study of 3-D transcapsule OCT imaging of intact lymph nodes demonstrating microstructural changes during metastatic infiltration. These results demonstrate the potential of OCT as a technique for intraoperative, real-time in situ 3-D optical biopsy of lymph nodes for the intraoperative staging of cancer.
- Willard-Mack CL. Normal structure, function, and histology of lymph nodes. Toxicol Pathol. 2006;34:409–24. CrossRef
- Torabi M, Aquino SL, Harisinghani MG. Current concepts in lymph node imaging. J Nucl Med. 2004;45:1509–18.
- Gadd M. Sentinel lymph node biopsy for staging early breast cancer: minimizing the trade-off by maximizing the accuracy. Ann Oncol. 2009;20:973–5. CrossRef
- Quan ML, McCready D. The evolution of lymph node assessment in breast cancer. J Surg Oncol. 2009;99:194–8. CrossRef
- Szabo BK, Aspelin P, Kristoffersen WM, Tot T, Bone B. Invasive breast cancer: correlation of dynamic mr features with prognostic factors. Eur Radiol. 2003;13:2425–35. CrossRef
- Fischbein NJ, Noworolski SM, Henry RG, Kaplan MJ, Dillon WP, Nelson SJ. Assessment of metastatic cervical adenopathy using dynamic contrast-enhanced MR imaging. Am J Neuroradiol. 2003;24:301–11.
- Kim SH, Kim SC, Choi BI, Han MC. Uterine cervical carcinoma: evaluation of pelvic lymph node metastasis with MR imaging. Radiology. 1994;190:807–11.
- Harisinghani MG, Barentsz J, Hahn PF, et al. Noninvasive detection of clinically occult lymph node metastases in prostate cancer. N Engl J Med. 2003;348:2491–9. CrossRef
- Anzai Y, Blackwell KE, Hirschowitz SL, et al. Initial clinical experience with dextran-coated superparamagnetic iron oxide for detection of lymph node metastases in patients with head and neck cancer. Radiology. 1994;192:709–15.
- Krag D, Weaver D, Ashikaga T, et al. The sentinel node in breast cancer—a multicenter validation study. N Engl J Med. 1998;339:941–6. CrossRef
- Blessing WD, Stolier AJ, Teng SC, Bolton JS, Fuhrman GM. A comparison of methylene blue and lymphazurin in breast cancer sentinel node mapping. Am J Surg. 2002;184:341–5. CrossRef
- Schaafsma BE, Mieog JS, Hutteman M, et al. The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery. J Surg Oncol. 2011;104:323–32. CrossRef
- Sampath L, Wang W, Sevick-Muraca EM. Near-infrared fluorescent optical imaging for nodal staging. J Biomed Opt. 2008;13:041312. CrossRef
- Rosbach KJ, Shin D, Muldoon TJ, et al. High-resolution fiber optic microscopy with fluorescent contrast enhancement for the identification of axillary lymph node metastases in breast cancer: a pilot study. Biomed Opt Express. 2010;1:911–22. CrossRef
- Nguyen NQ, Biankin AV, Leong RW, et al. Real time intraoperative confocal laser microscopy-guided surgery. Ann Surg. 2009;249:735–7. CrossRef
- Fujimoto JG, Brezinski ME, Tearney GJ, et al. Optical biopsy and imaging using optical coherence tomography. Nat Med. 1995:1:970–2. CrossRef
- Tearney GJ, Brezinski ME, Bouma BE, Boppart SA, Pitris C, Southern JF, Fujimoto JG. In vivo endoscopic optical biopsy with optical coherence tomography. Science. 1997;276:2037–9. CrossRef
- Boppart SA, Bouma BE, Pitris C, Southern JF, Brezinski ME, Fujimoto JG. In vivo cellular optical coherence tomography imaging. Nat Med. 1998;4:861–5. CrossRef
- Brezinski ME, Tearney GJ, Boppart SA, Swanson EA, Southern JF, Fujimoto JG. Optical biopsy with optical coherence tomography: feasibility for surgical diagnostics. J Surg Res. 1997;71:32–40. CrossRef
- Boppart SA, Herrmann JM, Pitris C, Stamper DL, Brezinski ME, Fujimoto JG. High-resolution optical coherence tomography guided laser ablation of surgical tissue. J Surg Res. 1999;82:275–84. CrossRef
- Boppart SA, Bouma BE, Pitris C, Tearney GJ, Southern JF, Brezinski ME, Fujimoto JG. Intraoperative assessment of microsurgery with three-dimensional optical coherence tomography. Radiology. 1998;208:81–6.
- Nguyen FT, Zysk AM, Chaney EJ, et al. Intraoperative evaluation of breast tumor margins with optical coherence tomography. Cancer Res. 2009;69:8790–6. CrossRef
- Luo W, Nguyen FT, Zysk AM, et al. Optical biopsy of lymph node morphology using optical coherence tomography. Tech Cancer Res Treat. 2005;4:539–47.
- McLaughlin RA, Scolaro L, Robbins P, Hamza S, Saunders C, Sampson DD. Imaging of human lymph nodes using optical coherence tomography: potential for staging cancer. Cancer Res. 2010;70:2579–84. CrossRef
- Nguyen FT, Zysk AM, Chaney EJ, et al. Optical coherence tomography: the intraoperative assessment of lymph nodes in breast cancer. IEEE Eng Med Biol. 2010;29:63–70. CrossRef
- Taback B, Hashimoto K, Kuo CT, Chan A, Giuliano AE, Hoon DS. Molecular lymphatic mapping of the sentinel lymph node. Am J Pathol. 2002;161:1153–61. CrossRef
- Klein T, Wieser W, Eigenwillig CM, Biedermann BR, Huber R. Megahertz OCT for ultrawide-field retinal imaging with a 1050 nm Fourier domain mode-locked laser. Opt Express. 2011;19:3044–62. CrossRef
- Tsai TH, Zhou C, Adler DC, Fujimoto JG. Frequency comb swept lasers. Opt Express. 2009;17:21257–70. CrossRef
- de Boer M, van Deurzen CHM, van Dijck JAAM, et al. Micrometastases or isolated tumor cells and the outcome of breast cancer. N Engl J Med. 2009;361:653–63. CrossRef
- Boppart SA, Bouma BE, Pitris C, Tearney GJ, Brezinski ME, Fujimoto JG. Forward-imaging instruments for optical coherence tomographic imaging. Opt Lett. 1997;22:1618–20. CrossRef
- Jung W, Kim J, Jeon M, Chaney EJ, Stewart CJ, Boppart SA. Handheld optical coherence tomography scanner for primary care diagnostics. IEEE Trans Biomed Eng. 2011;58:741–4. CrossRef
- Zysk AM, Nguyen FT, Chaney EJ, et al. Clinical feasibility of microscopically-guided breast needle biopsy using a fiber optic probe with computer-aided detection. Tech Cancer Res Treat. 2009;8:315–22.
- Li X, Chudoba C, Ko T, Pitris C, Fujimoto JG. Imaging needle for optical coherence tomography. Opt Lett. 2000;25:1520–2. CrossRef
- Three-dimensional Optical Coherence Tomography for Optical Biopsy of Lymph Nodes and Assessment of Metastatic Disease
Annals of Surgical Oncology
Volume 20, Issue 11 , pp 3685-3693
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- 1. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, USA
- 2. Department of Pathology, College of Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA
- 3. Departments of Bioengineering, Electrical and Computer Engineering, and Medicine, University of Illinois at Urbana-Champaign, Urbana, IL, USA