Annals of Surgical Oncology

, Volume 19, Supplement 3, pp 430–437

Clinical Significance of Molecular Detection of Matrix metalloproteinase-1 in Bone Marrow and Peripheral Blood in Patients with Gastric Cancer

  • Yoshimasa Kosaka
  • Koshi Mimori
  • Takeo Fukagawa
  • Kenji Ishikawa
  • Tsuyoshi Etoh
  • Hitoshi Katai
  • Takeshi Sano
  • Masahiko Watanabe
  • Mitsuru Sasako
  • Masaki Mori
Translational Research and Biomarkers

DOI: 10.1245/s10434-011-1905-y

Cite this article as:
Kosaka, Y., Mimori, K., Fukagawa, T. et al. Ann Surg Oncol (2012) 19: 430. doi:10.1245/s10434-011-1905-y

Abstract

Purpose

Matrix metalloproteinases are responsible for proteolytic degradation of basement membrane and extracellular matrix. In tumor tissues, elevated expression of matrix metalloproteinase-1 (MMP-1) has been associated with tumor invasion and metastasis. However, little is known about the expression of MMP-1 in peripheral blood (PB) and bone marrow (BM) in gastric cancer patients. Thus, the aim of the present study is to determine MMP-1 messenger RNA (mRNA) expression levels in BM and PB of patients with gastric cancer.

Methods

The study group consisted of 857 patients with gastric cancer (577 males and 280 females) ranging in age from 27 to 87 years (average 61.6 years). MMP-1 mRNA expression levels in BM and PB were evaluated quantitatively by real-time reverse-transcription polymerase chain reaction (RT-PCR).

Results

Expression of MMP-1 mRNA in BM and PB of patients with gastric cancer was significantly higher than in noncancer patients. High levels of MMP-1 mRNA expression were significantly associated with differentiated histology, tumor size, tumor invasiveness, lymph node metastasis, liver metastasis, and clinical stage. Particularly importantly, MMP-1 mRNA expression in PB was an independent factor of distant metastasis.

Conclusions

We disclosed that MMP-1 mRNA expression in peripheral blood and bone marrow of gastric cancer patients was very high, precisely reflecting staging of gastric cancer. MMP-1 mRNA expression in peripheral blood may be a useful marker for distant metastasis in gastric cancer.

Copyright information

© Society of Surgical Oncology 2011

Authors and Affiliations

  • Yoshimasa Kosaka
    • 1
    • 3
  • Koshi Mimori
    • 1
  • Takeo Fukagawa
    • 2
  • Kenji Ishikawa
    • 1
  • Tsuyoshi Etoh
    • 2
  • Hitoshi Katai
    • 2
  • Takeshi Sano
    • 2
  • Masahiko Watanabe
    • 3
  • Mitsuru Sasako
    • 2
  • Masaki Mori
    • 1
  1. 1.Department of Surgery and Molecular OncologyMedical Institute of Bioregulation, Kyushu UniversityBeppuJapan
  2. 2.Gastric Surgery DivisionNational Cancer Center HospitalChuo-kuJapan
  3. 3.Department of SurgeryKitasato University School of MedicineSagamiharaJapan