Translational Research and Biomarkers

Annals of Surgical Oncology

, Volume 18, Issue 6, pp 1775-1781

First online:

Tissue hOGG1 Genotype Predicts Bladder Cancer Prognosis: A Novel Approach Using a Peptide Nucleic Acid Clamping Method

  • Yun-Sok HaAffiliated withDepartment of Urology, College of Medicine, Chungbuk National University
  • , Chunri YanAffiliated withDepartment of Urology, College of Medicine, Chungbuk National University
  • , Isaac Yi KimAffiliated withSection of Urologic Oncology, The Cancer Institute of New Jersey, Robert Wood Johnson Medical School
  • , Seok-Joong YunAffiliated withDepartment of Urology, College of Medicine, Chungbuk National University
  • , Sung-Kwon MoonAffiliated withDepartment of Food and Biotechnology, Chungju National University
  • , Wun-Jae KimAffiliated withDepartment of Urology, College of Medicine, Chungbuk National University Email author 

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Abstract

Background

Tissue genotyping is a more useful approach than using blood genomic DNA, because the tumor tissues can reflect the effects of somatic mutations in cancer. We investigated the value of the human oxoguanine glycosylase (hOGG1) genotype determined in tumor tissues as a prognostic indicator for bladder cancer (BC) using a novel technological approach.

Methods

A total of 335 DNA samples from patients with primary BC were analyzed by peptide nucleic acid (PNA)-mediated real-time polymerase chain reaction (PCR) clamping to characterize the association between genetic polymorphisms within hOGG1 codon 326 and the clinicopathological characteristics of primary BC patients.

Results

Tumor stage and number were significantly associated with the hOGG1 codon 326 genotype in nonmuscle invasive bladder cancer (NMIBC) patients. Compared with Cys326Ser and Ser326Ser, the Cys326Cys genotype had a greater progression-free survival benefit in patients with muscle invasive bladder cancer (MIBC). Univariate and multivariate Cox regression analyses indicated that the hOGG1 Cys326Cys genotype has a protective effect against progression in MIBC (hazard ratio, 0.360 and 0.314, respectively).

Conclusions

The hOGG1 tissue genotype is associated with aggressive clinicopathological features in NMIBC and with progression in patients with MIBC. Results suggest that the hOGG1 tissue genotype represents a promising marker for assessing BC prognosis in the clinical setting.