Primary Hyperparathyroidism Patients with Positive Preoperative Sestamibi Scan and Negative Ultrasound Are More Likely to Have Posteriorly Located Upper Gland Adenomas (PLUGs)
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- Harari, A., Mitmaker, E., Grogan, R.H. et al. Ann Surg Oncol (2011) 18: 1717. doi:10.1245/s10434-010-1493-2
Standard preoperative imaging for primary hyperparathyroidism usually includes sestamibi scanning (MIBI) and ultrasound (US). In a subset of patients with a positive MIBI and a negative US, we hypothesize that the parathyroid adenomas are more likely to be located posteriorly in the neck, where anatomically they are more difficult to detect by US.
We retrospectively reviewed the records of 661 patients treated for primary hyperparathyroidism between 2004 and 2009 at a tertiary referral center. We included patients who for their first operation had a MIBI that localized a single lesion in the neck and an US that found no parathyroid adenoma. We excluded patients with persistent or recurrent hyperparathyroidism, and patients with MIBIs that were negative, that had more than one positive focus, or that had foci outside of the neck. Sixty-six cases were included in the final analysis.
A total of 54 patients (83%) had a single adenoma, 4 (6%) had double adenomas, and 7 (11%) had hyperplasia. Thirty-three patients (51%) had a single upper gland adenoma; 19 of these (58%) were posteriorly located upper gland adenomas (PLUGs). PLUGs occurred more often on the right side than on the left (P = 0.048, Fisher’s test). PLUGs were also larger than other single adenomas (mean 1.85 vs. 1.48 cm, P = 0.021, t-test). Seventy-six percent of patients successfully underwent a unilateral or focused exploration. Six patients (9%) had persistent disease, which is double our group’s overall average (4–5%).
Primary hyperparathyroid patients with preoperative positive MIBI and negative US are more likely to have PLUGs.
Primary hyperparathyroidism (PHPT) is a common cause of hypercalcemia, diagnosed in approximately 1 in 1000 people in the United States each year.1–3 The causes of PHPT include a single adenoma (80 to 85%), double adenomas (2 to 12%), hyperplasia (5 to 15%), or parathyroid carcinomas (<1%).2,4–7
The only curative treatment for PHPT is parathyroidectomy. Most parathyroid surgeons in the US use preoperative localization studies to guide a more focused operation and offer a unilateral minimally invasive surgical technique.7 If positive, this can limit the dissection to the abnormal parathyroid gland and thus substantially decrease operation time.8,9 For preoperative localization before first-time operations, most surgeons will order a 99mTc-sestamibi scan (MIBI) and an ultrasound (US) as their initial imaging studies.7
The sensitivity of MIBI for detection of solitary adenomas ranges from 68 to 95%. US carries a wide range of sensitivities (33 to 89%), depending on the experience of the sonographer, for the detection of solitary parathyroid adenomas.7,8,10–14 However, a preoperative approach that combines both the anatomic information of sonography and the physiologic information of scintigraphy has been shown to predict the presence and location of solitary adenomas more accurately than either technique alone. 8 When combined, the overall sensitivity can be increased up to 95%.7
Despite this, a subset of patients will have a positive MIBI and a negative US preoperatively before their first-time exploration for PHPT. In this case, because one of the studies is positive, most surgeons would go straight to surgery rather than order more imaging studies. Previous studies have not focused primarily on what a surgeon should expect to encounter intraoperatively with this specific combination of imaging results in the neck.
We hypothesized that in this subset of patients, the parathyroid adenomas are more likely to be located posteriorly in the neck, where anatomically they are more difficult to detect by US. Because, embryologically, descended upper parathyroid glands are located more posteriorly in the neck, we also hypothesized that most of these posterior lesions are descended upper parathyroid glands located in the tracheoesophageal groove or retroesophageally.
We retrospectively reviewed the records of all patients treated for PHPT between 2004 and 2009 from a prospective parathyroid database of a tertiary referral center. We included patients who underwent their first parathyroid operation, had a MIBI that localized only a single lesion in the neck, and had an US that found no parathyroid adenoma. We excluded patients with persistent, recurrent, secondary, or tertiary hyperparathyroidism. We also excluded any patients with MIBIs that were negative, that had more than one positive focus, or that had foci outside of the neck. All USs were performed by the radiology department of our hospital. The MIBI imaging results were not routinely available to the sonographers. For patients who had no prior localization studies, we routinely perform MIBI and US on the same day, but not in any particular sequence.
One of the two hospitals did not measure intraoperative parathyroid hormone (ioPTH) levels during this study period. We have previously shown that when both MIBI and US are positive and concordant, a focused exploration of just one gland without ioPTH has a similar success rate to the use of ioPTH. We also showed that when only one localizing study is positive, we should exclude hyperplasia by exploring one additional gland (unilateral exploration). Thus, the guidelines that the surgeons used when ioPTH was not available were: (1) if two localizing studies were positive, a focused exploration of one gland was performed; (2) if only one localizing study was positive, a unilateral exploration of both glands was performed to confirm that the other ipsilateral parathyroid gland was normal and to rule out hyperplasia; and (3) if there was no positive localizing study, a four-gland bilateral exploration was performed. We tend to use ioPTH aspirates of the excised glands to confirm parathyroid tissue. When ioPTH was not available, analysis of frozen sections was used to confirm pathology.
Single adenomas were defined as a single parathyroid adenoma found at the time of first operation. Upper or lower glands were designated by the surgeon at the time of operation, depending on their relative position to the recurrent laryngeal nerve. Glands posterior to the nerve were designated as upper glands. Those anterior to the nerve were designated as lower glands. Posteriorly located upper gland adenomas (PLUGs) were defined as those glands that were descended upper parathyroid glands located in the tracheoesophageal groove or in a retroesophageal location.
A double adenoma was defined as two confirmed hypercellular parathyroid glands, either found at the time of first operation or found at reoperation. If two diseased glands were excised and one was a PLUG, they were counted as double adenoma. Multigland disease was defined as three or four parathyroid adenomas found at the time of first operation or at reoperation.
Persistent hyperparathyroidism was defined as hypercalcemia encountered within six months of the first operation. The one exception was a patient with normocalcemic PHPT, in whom persistence was defined as persistently increased parathyroid hormone levels postoperatively. Most patients are followed by their referring endocrinologists after the postoperative clinic visit. Patients are instructed to have calcium level checked by the endocrinologists six months after surgery, then yearly, and to return to us if the levels are abnormal.
Average age (year)
Average body mass index
Average calcium level (preoperative)
10.79 mg/dl (n = 62)
Average calcium level (within 6 months after parathyroidectomy)
9.01 mg/dl (n = 65)
Average calcium level (>6 months after parathyroidectomy)
9.46 mg/dl (n = 21)
Average PTH level (preoperative)
139.4 pg/ml (n = 61)
Average PTH level (within 6 months after parathyroidectomy)
34.55 pg/ml (n = 64)
Average PTH level (>6 months after parathyroidectomy)
46.7 pg/ml (n = 15)
Average operating room time (patients with no concomitant thyroid operations)
Fifty-four patients (83%) had a single adenoma, 4 (6%) had double adenomas, and 7 (11%) had hyperplasia. No patients had parathyroid cancer. One patient who underwent bilateral neck exploration was excluded from the analysis of the number of glands causing PHPT because of excision of one lower gland with only mild hypercellularity, with no drop in ioPTH, and hence a known failure intraoperatively. The patient refused reoperation, and thus we cannot definitively conclude how many glands were causing his disease.
In those patients who had lower single glands adenomas found, 55% (11 of 20) had disease that was atypical in appearance or that was found in locations other than the normal position. The atypical areas included anterolateral/beneath the thyroid capsule (n = 1); thymus/thyrothymic ligament (n = 5); and posteromedial and associated with thyroid nodule (n = 1). The atypical appearances of the lower glands included bilobed appearance (n = 2); associated with multinodular goiter but in a normal location (n = 1); and wormlike appearance (n = 1).
Seventy-six percent of patients successfully underwent a unilateral or focused exploration. At last follow-up, the postoperative normocalcemia rate when ioPTH testing was used with a >50% drop was 92% (34 of 37). The normocalcemia rate with no use of ioPTH was 93% (26 of 28). Thus, use of ioPTH showed no difference in the rate of normocalcemia.
When looking at the appearance on MIBI, if it showed a single bright focus that was in the medial position, 46.6% were found intraoperatively to be PLUGs (7 of 15). Of the 19 PLUGs, 17 of them (89%) had midlevel (n = 6) or inferior (n =11) MIBI predictions. All patients in which PLUGs were found had a preoperative MIBI that showed a single focus in the medial position.
Type of operation
Percentage ioPTH drop
RL gland excised and right thyroid lobe taken; RU and LL never found
Patient refused reoperation
Posteromedial right upper
86% (slow drop; this delay was the reason for bilateral neck exploration)
PLUG found in area of MIBI prediction on right TE groove; right thyroid lobe taken as well (follicular neoplasm); RL never found; normal LU and LL observed
Lost to follow-up
RL and LL taken; RU and LU seemed normal
An additional gland was removed at a reoperation, so likely this was hyperplasia
Subtotal parathyroidectomy; left part of LL in situ
This patient had persistently increased PTH with normocalcemic hyperparathyroidism; no reoperation offered; investigating other causes of possible secondary hyperparathyroidism
Posterior left upper
LU and LL removed
Lost to follow-up, did not return for reoperation; likely had hyperplasia, given persistence after unilateral exploration only
RU PLUG taken as focused exploration
Lost to follow-up; likely had >1 gland diseased
MIBI predictions associated with multiple-gland diseasea
Type of disease
Double: RU and RL PT adenomas
PT hyperplasia (4-gland disease)
Double: LU and LL (intrathyroidal) PT adenomas
Posterior to left upper
PT hyperplasia (4-gland disease)
Double: RU and RL PT adenomas
PT hyperplasia; RU was a PLUG
PT hyperplasia (4-gland disease)
Right posterior inferior
PT 3-gland hyperplasia: LU, LL, RU PT involved
Left inferior (1/09)
Double: RL and LL PT adenomas
Double: Right PLUG, LL PT adenomas
PT hyperplasia (4-gland disease)
In this study, we found that patients with a single positive MIBI focus in the neck and a negative US have a similar distribution of gland pathology as the entire population of PHPT patients. However, patients with this particular combination of imaging studies have an unusually high incidence of posteriorly located upper glands (PLUGs).
Embryologically, upper gland adenomas are derived from the fourth branchial pouch.15 These upper glands tend to be more consistent in location than the lower glands with most (>90%) upper glands located deep in relation to the mid portion of the superior pole of the thyroid near the cricothyroid junction.8 Infrequently, the superior glands can be seen more inferiorly, deep in relation to the midpole of the thyroid lobes (4%), or they may be located at or above the most superior aspect of the thyroid (3%). Rarely, the superior glands can be found in the retropharyngeal (1%) or retroesophageal (1%) (2% PLUG) spaces, or in the thyroid gland itself (0.2%).8
In this investigation, we have shown that among the single upper glands found, there is a far higher than normal (58 vs. 2%, as noted above) proportion of these PLUGs when preoperative imaging shows a single positive MIBI focus in the neck and a negative US. Perrier et al. introduced a novel nomenclature to describe parathyroid adenoma location in the neck. If one uses their nomenclature, PLUGs would likely be labeled as B or C glands (those found in the tracheoesophageal groove).16
We should note that we excluded all patients with MIBI that revealed ectopic foci outside of the neck. Some of these patients will have MIBI that reveal mediastinal or undescended parathyroid adenomas. These patients will also have negative US. Our team looked closely at those patients with a single positive focus on MIBI in the neck only. Thus, it is important for the parathyroid surgeon to look closely at MIBI images themselves and recognize ectopic foci to understand how best to approach these tumors.
Berber et al.’s study evaluated 1000 patients in a prospective study to determine factors affecting negative localization with both MIBI and US.19 Their results concluded that as BMI increases, the sensitivity of US and of MIBI will decrease. Their explanation was that the increased amount of subcutaneous tissues would affect the ability of the sonographic waves to detect abnormal glands in the neck as well as cause difficulty in performing the examination. We also anticipated that BMI might adversely affect the ability of an US to see a PLUG, which is typically located deep in the neck. However, we found no statistical difference in BMI between those that had PLUGs versus those that were found to have other types of single adenomas. We have interpreted this to mean that even though an obese patient is more likely to have nonlocalizing US than a nonobese patient (per Siperstein), if an obese patient has a positive MIBI and negative US, he or she has the same chance of having a PLUG as a nonobese patient with the same findings.
In conclusion, primary hyperparathyroid patients with preoperative positive MIBI and negative US are more likely to have PLUGs. In this scenario, when an US is negative and a MIBI scan predicts disease in a lower quadrant, surgeons should not assume that a lower gland adenoma is the cause. One must look at the lateral planar views to determine the best approach because most of these will be upper parathyroid tumors that have descended unto the tracheoesophageal groove.
Conflict of interest
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