Laboratory Research

Annals of Surgical Oncology

, 15:2606

First online:

Prognostic Relevance of Tensin4 Expression in Human Gastric Cancer

  • Katsuya SakashitaAffiliated withDepartment of Surgical Oncology, Medical Institute of Bioregulation, Kyushu UniversityDepartment of Surgical Oncology, Osaka City University Graduate School of Medicine
  • , Koshi MimoriAffiliated withDepartment of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University Email author 
  • , Fumiaki TanakaAffiliated withDepartment of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University
  • , Yukio KamoharaAffiliated withDepartment of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University
  • , Hiroshi InoueAffiliated withDepartment of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University
  • , Tetsuji SawadaAffiliated withDepartment of Surgical Oncology, Osaka City University Graduate School of Medicine
  • , Kosei HirakawaAffiliated withDepartment of Surgical Oncology, Osaka City University Graduate School of Medicine
  • , Masaki MoriAffiliated withDepartment of Surgical Oncology, Medical Institute of Bioregulation, Kyushu University

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Abstract

Background

The Tensin4 gene is involved in various biological events by mediating signal transduction. This study was designed to clarify its clinical significance in gastric cancer.

Methods

A total of 114 gastric cancer patients were enrolled in this study, and we obtained paired samples from tumor tissue and matched normal mucosa. Total RNA was extracted, and TNS4 mRNA expression was quantified using real-time reverse-transcription polymerase chain reaction. TNS4 protein expression was investigated by immunohistochemistry. Additionally, the relationship between TNS4 expression and clinicopathologic factors was statistically analyzed.

Results

TNS4 mRNA expression was significantly higher in tumor tissue than in normal mucosa (p < 0.0001). Additional immunohistochemical analysis demonstrated that TNS4 protein was predominantly expressed in gastric cancer. Tumors with higher TNS4 mRNA expression showed histologically poorer grade (p < 0.02), deeper invasion into the serosa (p < 0.01), more positive lymph node metastasis (p < 0.02) or peritoneal dissemination (p < 0.05). Patients with high TNS4 mRNA expression tumor were more likely to have cancer-related death (p < 0.05). A Kaplan–Meier curve also demonstrated that patients with high TNS4 expression tumor had significantly poorer prognosis (p < 0.01) than those with low TNS4 expression tumor. In particular, the 2-year overall survival rates were 48.2% and 83.1%, respectively, a very significant difference. Multivariate analysis revealed that high TNS4 mRNA expression was an independent prognostic predictor (relative risk 1.41, 95% confidence interval 1.03–1.96, p = 0.03).

Conclusion

TNS4 was frequently overexpressed in gastric cancer, and tumors with high TNS4 mRNA expression showed biologically aggressive behavior. High TNS4 mRNA expression may be a novel prognostic predictor for those patients.

Keywords

TNS4 Gastric cancer Prognostic predictor