Annals of Surgical Oncology

, Volume 12, Issue 10, pp 800–807

Interleukin 8 in Human Hepatocellular Carcinoma Correlates With Cancer Cell Invasion of Vessels But Not With Tumor Angiogenesis

  • Fumitake Kubo
  • Shinichi Ueno
  • Kiyokazu Hiwatashi
  • Masahiko Sakoda
  • Koichi Kawaida
  • Kensuke Nuruki
  • Takashi Aikou
Article

DOI: 10.1245/ASO.2005.07.015

Cite this article as:
Kubo, F., Ueno, S., Hiwatashi, K. et al. Ann Surg Oncol (2005) 12: 800. doi:10.1245/ASO.2005.07.015

Abstract

Background

Angiogenic factor seems necessary for the development of hepatocellular carcinoma (HCC), which is a hypervascular malignancy. This study examined the expression of interleukin (IL)-8, a potent angiogenic factor, in HCC samples.

Methods

We measured IL−8 expression by using reverse transcriptase-polymerase chain reaction in clinical HCC tissues from 45 patients who underwent surgical resection. We then assessed correlations between IL−8 expression and microvessel growth or clinicopathologic factors. We also elucidated the in vitro effect of IL−8 on HepG2 development by using fluorometric assays of proliferation, chemotaxis, and invasion.

Results

The expression of IL−8 did not significantly correlate with the microvessel count in HCC tissues, but the incidence of microscopic vessel invasion was significantly higher in IL−8–positive than in IL−8–negative tissues. Thus, more IL−8 was expressed in HCCs at pathologic stage III/IV than in those at stage I/II. Assays in vitro showed that IL−8 stimulates HepG2 chemotactic and invasive activities rather than cell proliferation.

Conclusions

The expression of IL−8 in human HCC has more relevance to metastatic potential, such as vessel invasion, than to angiogenesis or cell proliferation.

Keywords

Interleukin 8Hepatocellular carcinomaTumor progressionAngiogenesis ChemotaxisVessel invasion.

Copyright information

© The Society of Surgical Oncology, Inc. 2005

Authors and Affiliations

  • Fumitake Kubo
    • 1
  • Shinichi Ueno
    • 1
  • Kiyokazu Hiwatashi
    • 1
  • Masahiko Sakoda
    • 1
  • Koichi Kawaida
    • 1
  • Kensuke Nuruki
    • 1
  • Takashi Aikou
    • 1
  1. 1.Course of Advanced Therapeutics, Field of Oncology, Department of Surgical Oncology and Digestive SurgeryKagoshimaUniversity Graduate School of Medicine and Dental SciencesKagoshimaJapan