Abstract
Cefuroxime axetil (CFA), an ester prodrug of cefuroxime exists as a pair of diastereoemers, namely isomer A and isomer B. To enable phase diagram construction, crystallization of the diastereomers of CFA from the commercially available amorphous drug substance was carried out. Isomer A was separated with a purity approaching 100% whereas the maximum purity of isomer B was 85% as confirmed by solution state proton NMR spectroscopy. The crystalline forms of isomer A and isomer B were confirmed as forms AI and BI, respectively, based on differential scanning calorimetry (DSC) analysis and powder X-ray diffraction. DSC analysis was used to observe the melting behavior of different diastereomer mixture compositions. The binary solid-liquid phase diagram for mixture compositions ranging from 0 to 85% w/w isomer B indicated the formation of a eutectic mixture having a melting temperature of 124.7 ± 0.4°C and a composition of 75% w/w (+/−5% wt.) isomer B. The eutectic composition was calculated using an index based on the van’t Hoff equation for melting point depression and was found to be 75% isomer B and 25% isomer A. As CFA is present in commercial preparations as a mixture of diastereomers, the formation of a eutectic mixture between the diastereomers may impact the solubility and stability of the commercial product. Eutectic formation can be explained on the basis of the chemical similarity of diastereomers that favor miscibility in the liquid state.
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References
Kees F, Lukassek U, Naber KG, Grobecker H. Comparative investigation on the bioavailability of cefuroxime axetil. Drug Res. 1991;41:843–6.
Mosher GL, McBee J, Shaw DB. Esterase activity towards diastereomers of cefuroxime axetil in the rat and dog. Pharm Res. 1992;9:687–9.
Stoeckel K, Hofheinz W, Laneury JP, Duchene P, Shedlofsky S, Blouin RA. Stability of cephalosporin prodrug esters in human intestinal juice: implications for oral bioavailability. Antimicrob Agents Chemother. 1998;42:2602–6.
Osczapowicz I, Malafiej E, Horoszewicz-Malafiej A, Szelachowska M, Kuklewicz C, Sieranska E. Esters of cephalosporins. Part III. Separation and properties of the R and S isomers of 1-acetoxyethyl ester of cefuroxime. Acta Pol Pharm. 1995;52:471–6.
Park A, Chyall LJ, Byrn S. Characterization of cefuroxime axetil. Glaxo Wellcome, SR-5597.01, SSCI Inc. 2000; West Lafayette, Indiana.
Li ZJ, Grant DJW. Relationship between the physical properties and crystal structures of chiral drugs. J Pharm Sci. 1997;86:1073–8.
Law D, Wang W, Schmitt EA, Long MA. Prediction of poly(ethylene) glycol-drug eutectic compositions using an index based on the van’t Hoff equation. Pharm Res. 2002;19:315–21.
Furuyama N, Hasegawa S, Yada S, Hamaura T, Wakiyama N, Yonemochi E, et al. Do amorphous troglitazone prepared from two diastereomer-pairs have the same molecular mobility and crystallization rate at the surface? Chem Pharm Bull. 2011;59(12):1452–7.
Bhatnagar BS, Cardon S, Pikal MJ, Bogner RH. Reliable determination of freeze-concentration using DSC. Thermochim Acta. 2005;425:149–63.
Kansal VK, Bhat SG. An improved method for preparation of cefuroxime axetil. 2004. EP 1 409 492 B1.
Mosher GL, Mullen MV. R-Cefuroxime axetil. 1991. U.S. Patent 5,063,224.
Bi M, Hwang S-J, Morris KR. Mechanism of eutectic formation upon compaction and its effect on tablet properties. Thermochim Acta. 2003;404:213–26.
Acknowledgements
The authors would like to thank the Mylan School of Pharmacy and the Duquesne University Faculty Development Fund (FDF) for providing financial support for this work. They would also like to thank Dhanuka Laboratories, Mumbai for donating the cefuroxime axetil used for this work.
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Dalal, N., Buckner, I.S. & Wildfong, P.L.D. Experimental Determination and Theoretical Calculation of the Eutectic Composition of Cefuroxime Axetil Diastereomers. AAPS PharmSciTech 18, 2570–2578 (2017). https://doi.org/10.1208/s12249-017-0739-8
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DOI: https://doi.org/10.1208/s12249-017-0739-8