Abstract
Three types of chitosan-based films have been prepared and evaluated: a non-modified chitosan film bearing cationizable aliphatic amines and two films made of N-sulfopropyl chitosan derivatives bearing both aliphatic amines and negative sulfonate groups at different ratios. Cell adhesion and proliferation on chitosan films of C2C12 pre-myoblastic cells and B16 cells as tumoral model have been tested. A differential cell behavior has been observed on chitosan films due to their different surface modification. B16 cells have shown lower vinculin expression when cultured on sulfonated chitosan films. This study shows how the interaction among cells and material surface can be modulated by physicochemical characteristics of the biomaterial surface, altering tumoral cell adhesion and proliferation processes.
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Acknowledgments
This paper was supported by the Spanish Ministry of Economy and Competitiveness (MAT 2010-20001 and CONSOLIDER CSD2009-00088 projects; JAR and IA JAE-PRE fellowship and JAE-DOC contract, respectively), the Spanish Ministry of Science and Innovation (SAF2011-27863 and PET2008_0168), and the Seventh Framework Program of the European Commission (EuroNanomed ERA-NET initiative, REBONE project; PI10/02985FIS). Dr. Carolina Garcia and Dr. Pilar Posada from Characterization Service (Institute of Polymer Science and Technology) are acknowledged for the AFM measurements and support.
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Guest Editors: Claudio Salomon, Francisco Goycoolea, and Bruno Moerschbacher
In Memoriam
In loving memory of our Professor José Luis López Lacomba, who taught us everything about science and life.
Enrique Martínez-Campos and Ana Civantos contributed equally to this work.
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Figure Suplemental 1
Cell proliferation over S-CS films with different degrees of sulfonation (0,3 and 0,7). A. Cell morphology at 72 h. B. Metabolic activity of C2C12 premioblasts and B16 tumoral cells measured with Alamar Blue, growing on S-CS films (0,3 and 0,7). The results are expressed as Relative Fluorescence Units (RFUs). (GIF 209 kb)
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Martínez-Campos, E., Civantos, A., Redondo, J.A. et al. Cell Adhesion and Proliferation on Sulfonated and Non-Modified Chitosan Films. AAPS PharmSciTech 18, 974–982 (2017). https://doi.org/10.1208/s12249-016-0619-7
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DOI: https://doi.org/10.1208/s12249-016-0619-7