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Sustained Anti-inflammatory Effect of Resveratrol-Phospholipid Complex Embedded Polymeric Patch

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ABSTRACT

Resveratrol-phospholipid complex (Phytosome®) (RSVP) was found better aqueous soluble and permeable than free resveratrol (RSV). RSVPs were incorporated in polymeric patch prepared by solvent casting method using Eudragit RL 100, PVP K30, and PEG 400 for application on dermal sites for sustained treating of inflammation. Prepared patches were evaluated for various physicochemical properties, surface morphology by SEM, TEM, and compatibility of patch components by FT-IR and DSC studies. Optimized formulation (F9) gave 95.79 ± 3.02% drug release and 51.36% (4.28 ± 0.48 mg/cm2) skin permeation after 24 h. Skin extract when examined for drug accumulation showed 38.31 ± 2.42% drug content. FE-SEM images of the patch taken after drug release and skin permeation studies showed that RSVPs in polymeric patch are stable and retain their structure after 24 h long exposure to physiologic environment. Sustained anti-inflammatory effect was established in carrageenan-induced paw edema model in which test formulation gave 84.10% inhibition of inflammation at 24 h as compared to 39.58% for standard diclofenac sodium gel. The CLSM study confirmed the localization of RSVPs for a longer period, thus enabling drug targeting to the dermis for sustained effect. Skin irritation test on rabbit revealed that the patches are safe for skin application. Histological observations suggested that after exposure to the permeants, the SC integrity had not altered and no evidence of presence of inflammatory cells found. RSVP (Phytosome®) containing patches abled to give sustained therapeutic effect that may be useful in treating acute and chronic inflammation.

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ACKNOWLEDGMENTS

The authors are thankful to SAIF, NEHU, Shillong (India), for allowing utilizing the SEM and TEM facility. We are thankful to IASST, Guwahati (India), for the access of FE-SEM facility. We are also thankful to the CIF of College of Veterinary Science, Guwahati. Further, the authors are thankful to the Guwahati Biotech Park, IIT Guwahati, for using the CLSM facility.

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Authors and Affiliations

  1. Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati, 781017, India

    Bhupen Kalita

  2. Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, 786004, India

    Bhupen Kalita & Malay K. Das

  3. Department of Anatomy and Histology, College of Veterinary Science, Guwahati, 781022, India

    Munmun Sarma & Anil Deka

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  1. Bhupen Kalita
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Kalita, B., Das, M.K., Sarma, M. et al. Sustained Anti-inflammatory Effect of Resveratrol-Phospholipid Complex Embedded Polymeric Patch. AAPS PharmSciTech 18, 629–645 (2017). https://doi.org/10.1208/s12249-016-0542-y

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