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Photodegradation of Fleroxacin Injection: II. Kinetics and Toxicological Evaluation

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Abstract

Photodegradation kinetics of fleroxacin were investigated in different injections. Five commercial formulations were analyzed before and after irradiation by determining residual volumes of fleroxacin with high-pressure liquid chromatography (HPLC), and different decomposition functions and models were obtained. Concentration levels of fleroxacin in injections caused the differences in photodegradation kinetics instead of ingredients. Influences of different pH values and presence of NaCl on photodegradation of fleroxacin were observed. Low pH value decreased the efficacy of photolysis and enhanced photostability of fleroxacin injections. Tentative structure of a new degradation product afforded was proposed. An acute toxicity assay using the bioluminescent bacterium Q67 was performed for fleroxacin injections after exposure to light. The research proved that fleroxacin was more photolabile in dilute injection, and acute toxicity of dilute injection increased more rapidly than that of concentrated injection during irradiation.

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Authors and Affiliations

  1. Department of Antibiotics, Anhui Institute for Food and Drug Control, Hefei, 230051, China

    Wei Li

  2. School of Medical Engineering, Hefei University of Technology, Hefei, 230009, China

    Jun Wang

  3. Department of Analytical Chemistry, China Pharmaceutical University, Nanjing, 210009, China

    Jun Wang & Yu-Zhu Hu

Authors
  1. Wei Li
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  2. Jun Wang
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  3. Yu-Zhu Hu
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Correspondence to Jun Wang.

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Li, W., Wang, J. & Hu, YZ. Photodegradation of Fleroxacin Injection: II. Kinetics and Toxicological Evaluation. AAPS PharmSciTech 14, 578–584 (2013). https://doi.org/10.1208/s12249-013-9942-4

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  • DOI: https://doi.org/10.1208/s12249-013-9942-4

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