Abstract
The L4 Global Harmonization Team on reagents and their stability focused on the management of critical reagents for pharmacokinetic, immunogenicity, and biomarker ligand binding assays. Regulatory guidance recognizes that reagents are important for ligand binding assays but do not address numerous aspects of critical reagent life cycle management. Reagents can be obtained from external vendors or developed internally, but regardless of their source, there are numerous considerations for their reliable long-term use. The authors have identified current best practices and provided recommendations for critical reagent lot changes, stability management, and documentation.
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Acknowledgments
We would like to acknowledge and thank the many scientists who provided feedback on the draft recommendations and completed the survey questionnaire. The feedback and information we received were very important in framing our final recommendations. We would also like to thank those who critically and carefully reviewed the final recommendations and manuscript.
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Guest Editors: Binodh DeSilva and Philip Timmerman
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King, L.E., Farley, E., Imazato, M. et al. Ligand Binding Assay Critical Reagents and Their Stability: Recommendations and Best Practices from the Global Bioanalysis Consortium Harmonization Team. AAPS J 16, 504–515 (2014). https://doi.org/10.1208/s12248-014-9583-x
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DOI: https://doi.org/10.1208/s12248-014-9583-x