The AAPS Journal

, Volume 15, Issue 4, pp 1128–1140

Mass Spectrometry-Based Targeted Proteomics as a Tool to Elucidate the Expression and Function of Intestinal Drug Transporters

Authors

    • Department of Clinical Pharmacology, Center of Drug Absorption and TransportUniversity Medicine Greifswald
  • Christian Gröer
    • Department of Clinical Pharmacology, Center of Drug Absorption and TransportUniversity Medicine Greifswald
  • Marek Drozdzik
    • Department of Experimental and Clinical PharmacologyPomeranian Medical University
  • Werner Siegmund
    • Department of Clinical Pharmacology, Center of Drug Absorption and TransportUniversity Medicine Greifswald
Review Article Theme: Targeted Proteomics Quantification for Membrane Proteins

DOI: 10.1208/s12248-013-9521-3

Cite this article as:
Oswald, S., Gröer, C., Drozdzik, M. et al. AAPS J (2013) 15: 1128. doi:10.1208/s12248-013-9521-3
Part of the following topical collections:
  1. Theme: Targeted Proteomics Quantification for Membrane Proteins

Abstract

Intestinal transporter proteins affect the oral bioavailability of many drugs in a significant manner. In order to estimate or predict their impact on oral drug absorption, data on their intestinal expression levels are needed. So far, predominantly mRNA expression data are available which are not necessarily correlated with the respective protein content. All available protein data were assessed by immunoblotting techniques such as Western blotting which both possess a number of limitations for reliable protein quantification. In contrast to this, mass spectrometry-based targeted proteomics may represent a promising alternative method to provide comprehensive protein expression data. In this review, we will summarize so far available intestinal mRNA and protein expression data for relevant human multidrug transporters. Moreover, recently observed mass spectrometry-based targeted proteomic data will be presented and discussed with respect to potential functional consequences. Associated to this, we will provide a short tutorial how to set up these methods and emphasize critical aspects in method development. Finally, potential limitations and pitfalls of this emerging technique will be discussed. From our perspective, LC-MS/MS-based targeted proteomics represents a valuable new method to comprehensively analyse the intestinal expression of transporter proteins. The resulting expression data are expected to improve our understanding about the intestinal processing of drugs.

KEY WORDS

drug transportersintestinalmass spectrometryquantificationtargeted proteomics

Copyright information

© American Association of Pharmaceutical Scientists 2013