The AAPS Journal

, 11:693

Gum Arabic-Coated Magnetic Nanoparticles for Potential Application in Simultaneous Magnetic Targeting and Tumor Imaging

  • Lei Zhang
  • Faquan Yu
  • Adam J. Cole
  • Beata Chertok
  • Allan E. David
  • Jingkang Wang
  • Victor C. Yang
Research Article

DOI: 10.1208/s12248-009-9151-y

Cite this article as:
Zhang, L., Yu, F., Cole, A.J. et al. AAPS J (2009) 11: 693. doi:10.1208/s12248-009-9151-y

Abstract

Magnetic iron oxide nanoparticles (MNP) coated with gum arabic (GA), a biocompatible phytochemical glycoprotein widely used in the food industry, were successfully synthesized and characterized. GA-coated MNP (GA-MNP) displayed a narrow hydrodynamic particle size distribution averaging about 100 nm; a GA content of 15.6% by dry weight; a saturation magnetization of 93.1 emu/g Fe; and a superparamagnetic behavior essential for most magnetic-mediated applications. The GA coating offers two major benefits: it both enhances colloidal stability and provides reactive functional groups suitable for coupling of bioactive compounds. In vitro results showed that GA-MNP possessed a superior stability upon storage in aqueous media when compared to commercial MNP products currently used in magnetic resonance imaging (MRI). In addition, significant cellular uptake of GA-MNP was evaluated in 9L glioma cells by electron spin resonance (ESR) spectroscopy, fluorescence microscopy, and MRI analyses. Based on these findings, it was hypothesized that GA-MNP might be utilized as a MRI-visible drug carrier in achieving both magnetic tumor targeting and intracellular drug delivery. Indeed, preliminary in vivo investigations validate this clinical potential. MRI visually confirmed the accumulation of GA-MNP at the tumor site following intravenous administration to rats harboring 9L glioma tumors under the application of an external magnetic field. ESR spectroscopy quantitatively revealed a 12-fold increase in GA-MNP accumulation in excised tumors when compared to contralateral normal brain. Overall, the results presented show promise that GA-MNP could potentially be employed to achieve simultaneous tumor imaging and targeted intra-tumoral drug delivery.

Key words

brain tumordrug deliverygum arabicmagnetic nanoparticlemagnetic targeting

Copyright information

© American Association of Pharmaceutical Scientists 2009

Authors and Affiliations

  • Lei Zhang
    • 1
    • 2
    • 3
  • Faquan Yu
    • 3
  • Adam J. Cole
    • 3
  • Beata Chertok
    • 3
  • Allan E. David
    • 3
  • Jingkang Wang
    • 1
    • 2
  • Victor C. Yang
    • 1
    • 2
    • 3
  1. 1.Tianjin Key Laboratory for Modern Drug Delivery and High EfficiencyTianjin UniversityTianjinChina
  2. 2.School of Chemical EngineeringTianjin UniversityTianjinChina
  3. 3.Department of Pharmaceutical Sciences, College of PharmacyUniversity of MichiganAnn ArborUSA