The AAPS Journal

, Volume 10, Issue 3, pp 455–472

CNS Delivery Via Adsorptive Transcytosis

  • Françoise Hervé
  • Nicolae Ghinea
  • Jean-Michel Scherrmann
Review Article/Theme: Advances in CNS Delivery of Therapeutic Molecules/Guest Editors: J-M. Scherrmann and C. Svensson Themed Issue: Current Advances in Central Nervous System Delivery of Therapeutic Molecules Guest Editors: Jean-Michel Scherrmann

DOI: 10.1208/s12248-008-9055-2

Cite this article as:
Hervé, F., Ghinea, N. & Scherrmann, JM. AAPS J (2008) 10: 455. doi:10.1208/s12248-008-9055-2


Adsorptive-mediated transcytosis (AMT) provides a means for brain delivery of medicines across the blood-brain barrier (BBB). The BBB is readily equipped for the AMT process: it provides both the potential for binding and uptake of cationic molecules to the luminal surface of endothelial cells, and then for exocytosis at the abluminal surface. The transcytotic pathways present at the BBB and its morphological and enzymatic properties provide the means for movement of the molecules through the endothelial cytoplasm. AMT-based drug delivery to the brain was performed using cationic proteins and cell-penetrating peptides (CPPs). Protein cationization using either synthetic or natural polyamines is discussed and some examples of diamine/polyamine modified proteins that cross BBB are described. Two main families of CPPs belonging to the Tat-derived peptides and Syn-B vectors have been extensively used in CPP vector-mediated strategies allowing delivery of a large variety of small molecules as well as proteins across cell membranes in vitro and the BBB in vivo. CPP strategy suffers from several limitations such as toxicity and immunogenicity—like the cationization strategy—as well as the instability of peptide vectors in biological media. The review concludes by stressing the need to improve the understanding of AMT mechanisms at BBB and the effectiveness of cationized proteins and CPP-vectorized proteins as neurotherapeutics.

Key words

adsorptive-mediated transcytosis blood-brain barrier cationic proteins cell-penetrating peptides 

Copyright information

© American Association of Pharmaceutical Scientists 2008

Authors and Affiliations

  • Françoise Hervé
    • 1
  • Nicolae Ghinea
    • 2
  • Jean-Michel Scherrmann
    • 3
  1. 1.UFR BiomédicaleUniversité Paris DescartesParisFrance
  2. 2.Faculté de MédecineUniversité Paris 12CréteilFrance
  3. 3.INSERM, U705, CNRS, UMR7157, Hôpital Fernand Widal, Université Paris DescartesUniversité Paris DiderotParisFrance

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