AAPS PharmSciTech

, Volume 4, Issue 3, pp 24–35

Preparation and characterization of novel coenzyme Q10 nanoparticles engineered from microemulsion precursors

  • Cheng-Hsuan Hsu
  • Zhengrong Cui
  • Russell J. Mumper
  • Michael Jay
Article

DOI: 10.1208/pt040332

Cite this article as:
Hsu, CH., Cui, Z., Mumper, R.J. et al. AAPS PharmSciTech (2003) 4: 24. doi:10.1208/pt040332

Abstract

The purpose of these studies was to prepare and characterize nanoparticles into which Coenzyme Q10 (CoQ10) had been incorporated (CoQ10-NPs) using a simple and potentially scalable method. CoQ10-NPs were prepared by cooling warm microemulsion precursors composed of emulsifying wax, CoQ10, Brij 78, and/or Tween 20. The nanoparticles were lyophilized, and the stability of CoQ10-NPs in both lyophilized form and aqueous suspension was monitored over 7 days. The release of CoQ10 from the nanoparticles was investigated at 37°C. Finally, an in vitro study of the uptake of CoQ10-NPs by mouse macrophage, J774A.1, was completed. The incorporation efficiency of CoQ10 was approximately 74%±5%. Differential Scanning Calorimetry (DSC) showed that the nanoparticle was not a physical mixture of its individual components. The size of the nanoparticles increased over time if stored in aqueous suspension. However, enhanced stability was observed when the nanoparticles were stored at 4°C. Storage in lyophilized form demonstrated the highest stability. The in vitro release profile of CoQ10 from the nanoparticles showed an initial period of rapid release in the first 9 hours followed by a period of slower and extended release. The uptake of CoQ10-NPs by the J774A.1 cells was over 4-fold higher than that of the CoQ10-free nanoparticles (P<.05). In conclusion, CoQ10-NPs with potential application for oral CoQ10 delivery were engineered readily from microemulsion precursors.

Keywords

Coenzyme Q10nanoparticlesmicroemulsionstabilitycell uptake

Copyright information

© American Association of Pharmaceutical Scientists 2003

Authors and Affiliations

  • Cheng-Hsuan Hsu
    • 1
  • Zhengrong Cui
    • 1
  • Russell J. Mumper
    • 1
  • Michael Jay
    • 1
  1. 1.Division of Pharmaceutical Sciences, College of PharmacyUniversity of KentuckyLexington