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Novel pH- and Temperature-Responsive Blend Hydrogel Microspheres of Sodium Alginate and PNIPAAm-g-GG for Controlled Release of Isoniazid

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Abstract

This paper reports the preparation and characterization of novel pH- and thermo-responsive blend hydrogel microspheres of sodium alginate (NaAlg) and poly(N-isopropylacrylamide)(PNIPAAm)-grafted-guar gum (GG) i.e., PNIPAAm-g-GG by emulsion cross-linking method using glutaraldehyde (GA) as a cross-linker. Isoniazid (INZ) was chosen as the model antituberculosis drug to achieve encapsulation up to 62%. INZ has a plasma half-life of 1.5 h, whose release was extended up to 12 h. Fourier transform infrared spectroscopy was used to confirm the grafting reaction and chemical stability of INZ during the encapsulation. Differential scanning calorimetry was used to investigate the drug’s physical state, while powder X-ray diffraction confirmed the molecular level dispersion of INZ in the matrix. Scanning electron microscopy confirmed varying surface morphologies of the drug-loaded microspheres. Temperature- and pH-responsive nature of the blend hydrogel microspheres were investigated by equilibrium swelling, and in vitro release experiments were performed in pH 1.2 and pH 7.4 buffer media at 37°C as well as at 25°C. Kinetics of INZ release was analyzed by Ritger–Peppas empirical equation to compute the diffusional exponent parameter (n), whose value ranged between 0.27 and 0.58, indicating the release of INZ follows a diffusion swelling controlled release mechanism.

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ACKNOWLEDGMENT

The authors (Mr. P.B. Kajjari and Professor. L.S. Manjeshwar) thank the University Grants Commission (UGC), New Delhi, India (KU/SHA/UGC/RFSMS/2009-10), for a fellowship to P.B. Kajjari. Professor T. M. Aminabhavi thanks the All India Council for Technical Education, New Delhi [F.NO. 1-51/RIFD/EF(13)/2011-12] for Emeritus Fellowship.

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Authors and Affiliations

  1. Department of Chemistry, Karnatak University, Dharwad, 580 003, India

    Praveen B. Kajjari & Lata S. Manjeshwar

  2. All Indian Council for Technical Education, SET’s College of Pharmacy, Dharwad, 580002, India

    Tejraj M. Aminabhavi

Authors
  1. Praveen B. Kajjari
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  2. Lata S. Manjeshwar
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  3. Tejraj M. Aminabhavi
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Correspondence to Lata S. Manjeshwar.

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Kajjari, P.B., Manjeshwar, L.S. & Aminabhavi, T.M. Novel pH- and Temperature-Responsive Blend Hydrogel Microspheres of Sodium Alginate and PNIPAAm-g-GG for Controlled Release of Isoniazid. AAPS PharmSciTech 13, 1147–1157 (2012). https://doi.org/10.1208/s12249-012-9838-8

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  • DOI: https://doi.org/10.1208/s12249-012-9838-8

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