Research

Stem Cell Research & Therapy

, 4:41

Open Access This content is freely available online to anyone, anywhere at any time.

The dual effect of mscs on tumour growth and tumour angiogenesis

  • Michelle KéramidasAffiliated withInserm U823, Institut Albert Bonniot, Université Joseph Fourier
  • , Florence de FraipontAffiliated withInserm U823, Institut Albert Bonniot, Université Joseph FourierUM Biochimie des Cancers et Biothérapies, CHU de Grenoble, Institut de Biologie et Pathologie, Parvis Belledonne
  • , Anastassia KarageorgisAffiliated withInserm U823, Institut Albert Bonniot, Université Joseph Fourier
  • , Anaïck MoisanAffiliated withInserm U836, Grenoble Institut des Neurosciences, Université Joseph Fourier, Chemin Fortuné Ferrini
  • , Virginie PersoonsAffiliated withUM Biochimie des Cancers et Biothérapies, CHU de Grenoble, Institut de Biologie et Pathologie, Parvis Belledonne
  • , Marie-Jeanne RichardAffiliated withInserm U823, Institut Albert Bonniot, Université Joseph FourierUM Biochimie des Cancers et Biothérapies, CHU de Grenoble, Institut de Biologie et Pathologie, Parvis Belledonne
  • , Jean-Luc CollAffiliated withInserm U823, Institut Albert Bonniot, Université Joseph Fourier Email author 
  • , Claire RomeAffiliated withInserm U823, Institut Albert Bonniot, Université Joseph Fourier Email author 

Abstract

Introduction

Understanding the multiple biological functions played by human mesenchymal stem cells (hMSCs) as well as their development as therapeutics in regenerative medicine or in cancer treatment are major fields of research. Indeed, it has been established that hMSCs play a central role in the pathogenesis and progression of tumours, but their impact on tumour growth remains controversial.

Methods

In this study, we investigated the influence of hMSCs on the growth of pre-established tumours. We engrafted nude mice with luciferase-positive mouse adenocarcinoma cells (TSA-Luc+) to obtain subcutaneous or lung tumours. When tumour presence was confirmed by non-invasive bioluminescence imaging, hMSCs were injected into the periphery of the SC tumours or delivered by systemic intravenous injection in mice bearing either SC tumours or lung metastasis.

Results

Regardless of the tumour model and mode of hMSC injection, hMSC administration was always associated with decreased tumour growth due to an inhibition of tumour cell proliferation, likely resulting from deep modifications of the tumour angiogenesis. Indeed, we established that although hMSCs can induce the formation of new blood vessels in a non-tumoural cellulose sponge model in mice, they do not modify the overall amount of haemoglobin delivered into the SC tumours or lung metastasis. We observed that these tumour vessels were reduced in number but were longer.

Conclusions

Our results suggest that hMSCs injection decreased solid tumour growth in mice and modified tumour vasculature, which confirms hMSCs could be interesting to use for the treatment of pre-established tumours.