, 8:P191
Date: 15 Mar 2004

New diagnostic marker for sepsis: soluble CD14 subtype

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Introduction

Accurate and timely diagnosis of sepsis is essential for the treatment of the early infected patients of systemic inflammatory response syndrome (SIRS). But current diagnostic methods for early detection and for detection of the severity of sepsis have low sensitivity and poor specificity. This study aimed to develop a new diagnostic marker, serum soluble CD14 subtype(sCD14-ST), which was first discovered as a small molecular weight of CD14 specific for sepsis.

Methods

A small molecular weight of CD14 was discovered by a new type of soluble CD14 ELISA, which was developed with two types of anti-CD14 antibodies and evaluated using serum samples of patient with sepsis, SIRS, other diseases and healthy control. At the same time, the determination of the C-reactive protein, endotoxin, soluble CD14 (IBL-Hamburg), procalcitonin (PCT) (BRAHMS) and SOFA scoring were also carried.

Results

The new assay detected the small molecular weight of CD14 specifically, but not the 49 kDa and 55 k