TGF beta1 and related-Smads contribute to pulmonary metastasis of hepatocellular carcinoma in mice model
Recent studies indicate that Transforming Growth Factor beta (TGF β) correlated with pulmonary metastasis of cancers. However, the correlation between TGF β and pulmonary metastasis of hepatocellular carcinoma (HCC) is till unknown.
We detected the in vitro and in vivo expression levels of TGF β1/Smads by Real-time PCR and Western blot in MHCC97-H and MHCC97–L cell lines, which are HCC cell lines and have higher and lower pulmonary metastatic potential respectively.
TGF β1 mRNA level in MHCC97-L tumors were higher than that in MHCC97-H tumors, (2.81±1.61 vs. 1.24±0.96, P=0.002), TGF β1 protein level in MHCC97-L tumors were also higher than that in MHCC97-H tumors (1.37±0.95 vs. 0.32±0.22, P<0.001). In addition, the TGF β1 mRNA level positively correlated with pulmonary metastasis, and the relations between TGF β1 and Smads were also found (R2=0.12 and 0.40, respectively).
Our results suggest that TGF β/ Smads promote pulmonary metastasis of HCC.
- TGF beta1 and related-Smads contribute to pulmonary metastasis of hepatocellular carcinoma in mice model
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
Journal of Experimental & Clinical Cancer Research
- Online Date
- November 2012
- Online ISSN
- BioMed Central
- Additional Links
- Hepatocellular carcinoma
- Transforming growth factor beta
- Author Affiliations
- 1. Liver Cancer Institute & Zhongshan Hospital, Institutes of Biomedical Science, Fudan University, Shanghai, China
- 2. GaoXin Hospital, Xi’an JiaoTong University, Xi’an, Shanxi Province, China