Research

Head & Face Medicine

, 8:25

Open Access This content is freely available online to anyone, anywhere at any time.

An in vitro study of osteoblast vitality influenced by the vitamins C and E

  • Kent UrbanAffiliated withDepartment of Cranio-Maxillofacial Surgery, University of Münster
  • , Hans J HöhlingAffiliated withInstitute of Medical Physics and Biophysics, University of Münster
  • , Beate LüttenbergAffiliated withDepartment of Cranio-Maxillofacial Surgery, University of Münster
  • , Thomas SzuwartAffiliated withDepartment of Cranio-Maxillofacial Surgery, University of Münster
  • , Ulrich PlateAffiliated withDepartment of Cranio-Maxillofacial Surgery, University of Münster Email author 
  • , Biomineralisation Research Unit

Abstract

Vitamin C and vitamin E are known as important cellular antioxidants and are involved in several other non-antioxidant processes. Generally vitamin C and vitamin E are not synthesized by humans and therefore have to be applied by nutrition. The absence or deficiency of the vitamins can lead to several dysfunctions and even diseases (e.g. scurvy). The main interest in this study is that vitamin C and E are known to influence bone formation, e.g. vitamin C plays the key role in the synthesis of collagen, the major component of the extracellular bone matrix.

In the present study we evaluate the effect of ascorbic acid (vitamin C) and α-tocopherol (vitamin E) on the proliferation and differentiation of primary bovine osteoblasts in vitro. Starting from standard growth medium we minimized the foetal calf serum to reduce their stimulatory effect on proliferation.

An improved growth and an increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin was observed while increasing the ascorbic acid concentration up to 200 μg/ml. Furthermore the effects of α-tocopherol on cell growth and cell differentiation were examined, whereby neither improved growth nor increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin were detected.

Further investigations are necessary to target at better supportive effect of vitamins on bone regeneration, and healing.

Keywords

Bone cells Osteoblast Ascorbic acid Tocopherol Bone regeneration