Research

Diagnostic Pathology

, 7:135

Open Access This content is freely available online to anyone, anywhere at any time.

Ezrin overexpression predicts the poor prognosis of gastric adenocarcinoma

  • Jingchun JinAffiliated withDepartment of Pathology, Yanbian University Medical CollegeDepartment of Internal Medicine, Yanbian University Affiliated Hospital Email author 
  • , Tiefeng JinAffiliated withDepartment of Pathology, Yanbian University Medical CollegeCancer Research Center, Yanbian University Email author 
  • , Meiling QuanAffiliated withDepartment of Pathology, Yanbian University Medical CollegeCancer Research Center, Yanbian University
  • , Yingshi PiaoAffiliated withCancer Research Center, Yanbian UniversityDepartment of Pathophysiology, Yanbian University Medical College
  • , Zhenhua LinAffiliated withDepartment of Pathology, Yanbian University Medical CollegeCancer Research Center, Yanbian University Email author 

Abstract

Background

Ezrin is a cytoskeletal protein that is involved in tumor growth and invasion. It has been suggested that Ezrin expression plays an important role in tumor metastasis. This study is aimed to investigate the clinicopathological significance of Ezrin overexpression in gastric adenocarcinomas.

Methods

Ezrin protein expression was examined by immunohistochemistry in 26 normal gastric mucosa, 32 dysplasia, and 277 gastric adenocarcinomas. The relationship between Ezrin expression and the clinicopathological features of gastric cancers was analyzed. In addition, a gastric cancer cell line, MKN-1, was also used for immunofluorescence staining to evaluate the distribution of Ezrin protein.

Results

Ezrin protein located in the cytoplasm and/or membrane in the migrating gastric cancer cells, and it mainly concentrated at the protrusion site; however, only cytoplasmic distribution was observed in the non-migrating cancer cells by immunofluorescence staining. The positive rate of Ezrin protein expression was significantly higher in gastric adenocarcinoma and dysplasia compared with that in the normal gastric mucosa. Moreover, expression frequency of Ezrin protein increased significantly in lymph node metastasis and late clinical stages. Consistently, strong expression of Ezrin was significantly correlated with poor prognosis of gastric cancer.

Conclusion

The detection of Ezrin expression can be used as the marker for early diagnosis and prognosis of gastric adenocarcinoma.

Virtual Slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/2303598677653946

Keywords

Gastric adenocarcinoma Ezrin Tissue microarray