Research

Reproductive Biology and Endocrinology

, 10:71

Open Access This content is freely available online to anyone, anywhere at any time.

Steroidogenic and maturation-inducing potency of native gonadotropic hormones in female chub mackerel, Scomber japonicus

  • Hirofumi OhgaAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Kensuke KanekoAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Akio ShimizuAffiliated withNational Research Institute of Fisheries Science, Fisheries Research Agency
  • , Hajime KitanoAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Sethu SelvarajAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Mitsuo NyujiAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Hayato AdachiAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Akihiko YamaguchiAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University
  • , Michiya MatsuyamaAffiliated withLaboratory of Marine Biology, Faculty of Agriculture, Kyushu University Email author 

Abstract

Background

The gonadotropins (GtHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are produced in the pituitary gland and regulates gametogenesis through production of gonadal steroids. However, respective roles of two GtHs in the teleosts are still incompletely characterized due to technical difficulties in the purification of native GtHs.

Methods

Native FSH and LH were purified from the pituitaries of adult chub mackerel, Scomber japonicus by anion-exchange chromatography and immunoblotting using specific antisera. The steroidogenic potency of the intact chub mackerel FSH (cmFSH) and LH (cmLH) were evaluated in mid- and late-vitellogenic stage follicles by measuring the level of gonadal steroids, estradiol-17beta (Ε2) and 17,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P). In addition, we evaluated the maturation-inducing potency of the GtHs on same stage follicles.

Results

Both cmFSH and cmLH significantly stimulated E2 production in mid-vitellogenic stage follicles. In contrast, only LH significantly stimulated the production of 17,20beta-P in late-vitellogenic stage follicles. Similarly, cmLH induced final oocyte maturation (FOM) in late-vitellogenic stage follicles.

Conclusions

Present results indicate that both FSH and LH may regulate vitellogenic processes, whereas only LH initiates FOM in chub mackerel.

Keywords

Chub mackerel Perciform Asynchronous FSH LH Steroidogenesis Oocyte maturation