Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
The extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity.
Mouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM.
The cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells.
Mechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts.
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- Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
BioMedical Engineering OnLine
- Online Date
- October 2012
- Online ISSN
- BioMed Central
- Additional Links
- Tensile strain
- Extracellular matrix
- Author Affiliations
- 1. Academy of Military Medical Science, Tianjin Institute of Medical Equipment, No 106 Wandong Road, Hedong District, Tianjin, 300161, China
- 2. School of Mechanical Engineering, Tianjin University of Technology, No. 263 Hongqi Nalu Road, Nankai District, Tianjin, 300191, China
- 3. Shandong provincial Key lab of biophysics, Dezhou University, No. 566 Daxuexi Road, Decheng District, Dezhou, 253021, China