Use of inert gas jets to measure the forces required for mechanical gene transfection
- Guillaume Chouinard-PelletierAffiliated withChemical Engineering Department, McGill University
- , Mathieu LeducAffiliated withChemical Engineering Department, McGill University
- , David GuayAffiliated withChemical Engineering Department, McGill University
- , Sylvain CoulombeAffiliated withChemical Engineering Department, McGill University
- , Richard L LeaskAffiliated withChemical Engineering Department, McGill UniversityMontreal Heart Institute
- , Elizabeth AV JonesAffiliated withChemical Engineering Department, McGill UniversityLady Davis Institute, Jewish General Hospital, McGill University Email author
Transferring genes and drugs into cells is central to how we now study, identify and treat diseases. Several non-viral gene therapy methods that rely on the mechanical disruption of the plasma membrane have been proposed, but the success of these methods has been limited due to a lack of understanding of the mechanical parameters that lead to cell membrane permeability.
We use a simple jet of inert gas to induce local transfection of plasmid DNA both in vitro (HeLa cells) and in vivo (chicken chorioallantoic membrane). Five different capillary tube inner diameters and three different gases were used to treat the cells to understand the dependency of transfection efficiency on the dynamic parameters.
The simple setup has the advantage of allowing us to calculate the forces acting on cells during transfection. We found permeabilization efficiency was related to the dynamic pressure of the jet. The range of dynamic pressures that led to transfection in HeLa cells was small (200 ± 20 Pa) above which cell stripping occurred. We determined that the temporary pores allow the passage of dextran up to 40 kDa and reclose in less than 5 seconds after treatment. The optimized parameters were also successfully tested in vivo using the chorioallantoic membrane of the chick embryo.
The results show that the number of cells transfected with the plasmid scales with the dynamic pressure of the jet. Our results show that mechanical methods have a very small window in which cells are permeabilized without injury (200 to 290 Pa). This simple apparatus helps define the forces needed for physical cell transfection methods.
KeywordsTransfection Non-viral gene therapy Naked plasmid DNA Gene expression
- Use of inert gas jets to measure the forces required for mechanical gene transfection
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
BioMedical Engineering OnLine
- Online Date
- September 2012
- Online ISSN
- BioMed Central
- Additional Links
- Non-viral gene therapy
- Naked plasmid DNA
- Gene expression
- Author Affiliations
- 1. Chemical Engineering Department, McGill University, 3610 rue University, Montréal, H3A 2B2, Canada
- 2. Montreal Heart Institute, Montréal, H1T 1C8, Canada
- 3. Lady Davis Institute, Jewish General Hospital, McGill University, 3755 Cote Ste- Catherine Rd, Montreal, QC, H3T 1E2, Canada