Paralemmin-1 is over-expressed in estrogen-receptor positive breast cancers
Paralemmin-1 is a phosphoprotein lipid-anchored to the cytoplasmic face of membranes where it functions in membrane dynamics, maintenance of cell shape, and process formation. Expression of paralemmin-1 and its major splice variant (Δ exon 8) as well as the extent of posttranslational modifications are tissue- and development-specific. Paralemmin-1 expression in normal breast and breast cancer tissue has not been described previously.
Paralemmin-1 mRNA and protein expression was evaluated in ten breast cell lines, 26 primary tumors, and 10 reduction mammoplasty (RM) tissues using real time RT-PCR. Paralemmin-1 splice variants were assessed in tumor and RM tissues using a series of primers and RT-PCR. Paralemmin-1 protein expression was examined in cell lines using Western Blots and in 31 ductal carcinomas in situ, 65 infiltrating ductal carcinomas, and 40 RM tissues using immunohistochemistry. Paralemmin-1 mRNA levels were higher in breast cancers than in RM tissue and estrogen receptor (ER)-positive tumors had higher transcript levels than ER-negative tumors. The Δ exon 8 splice variant was detected more frequently in tumor than in RM tissues. Protein expression was consistent with mRNA results showing higher paralemmin-1 expression in ER-positive tumors.
The differential expression of paralemmin-1 in a subset of breast cancers suggests the existence of variation in membrane dynamics that may be exploited to improve diagnosis or provide a therapeutic target.
- Paralemmin-1 is over-expressed in estrogen-receptor positive breast cancers
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
Cancer Cell International
- Online Date
- May 2012
- Online ISSN
- BioMed Central
- Additional Links
- Breast cancer
- Estrogen receptor
- Tissue microarrays
- Splice variants
- Author Affiliations
- 1. University of Massachusetts, 637 North Pleasant Street, Amherst, MA, 01003-9298, USA
- 2. Pioneer Valley Life Sciences Institute, 3601 Main Street, Springfield, MA, 01107, USA
- 3. Department of Pathology, Baystate Medical Center, Springfield, MA, 01199, USA
- 4. Department of Neuroscience, Uppsala University Biomedical Center, Uppsala, S-75124, Sweden