Cell proliferation along vascular islands during microvascular network growth
- Molly R Kelly-GossAffiliated withDepartment of Biomedical Engineering, Tulane University
- , Erica R WintererAffiliated withDepartment of Biomedical Engineering, Tulane University
- , Peter C StaporAffiliated withDepartment of Biomedical Engineering, Tulane University
- , Ming YangAffiliated withDepartment of Biomedical Engineering, Tulane University
- , Richard S SweatAffiliated withDepartment of Biomedical Engineering, Tulane University
- , William B StallcupAffiliated withSanford-Burnham Medical Research Institute
- , Geert W Schmid-SchönbeinAffiliated withDepartment of Bioengineering, University of California-San Diego
- , Walter L MurfeeAffiliated withDepartment of Biomedical Engineering, Tulane University Email author
Observations in our laboratory provide evidence of vascular islands, defined as disconnected endothelial cell segments, in the adult microcirculation. The objective of this study was to determine if vascular islands are involved in angiogenesis during microvascular network growth.
Mesenteric tissues, which allow visualization of entire microvascular networks at a single cell level, were harvested from unstimulated adult male Wistar rats and Wistar rats 3 and 10 days post angiogenesis stimulation by mast cell degranulation with compound 48/80. Tissues were immunolabeled for PECAM and BRDU. Identification of vessel lumens via injection of FITC-dextran confirmed that endothelial cell segments were disconnected from nearby patent networks. Stimulated networks displayed increases in vascular area, length density, and capillary sprouting. On day 3, the percentage of islands with at least one BRDU-positive cell increased compared to the unstimulated level and was equal to the percentage of capillary sprouts with at least one BRDU-positive cell. At day 10, the number of vascular islands per vascular area dramatically decreased compared to unstimulated and day 3 levels.
These results show that vascular islands have the ability to proliferate and suggest that they are able to incorporate into the microcirculation during the initial stages of microvascular network growth.
KeywordsAngiogenesis Microcirculation Mesentery Proliferation Endothelial cell
- Cell proliferation along vascular islands during microvascular network growth
- Open Access
- Available under Open Access This content is freely available online to anyone, anywhere at any time.
- Online Date
- June 2012
- Online ISSN
- BioMed Central
- Additional Links
- Endothelial cell
- Author Affiliations
- 1. Department of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA
- 2. Sanford-Burnham Medical Research Institute, La Jolla, CA, 92037, USA
- 3. Department of Bioengineering, University of California-San Diego, LA Jolla, CA, 92093-0412, USA