Skip to main content
Log in

Status of the crystallography beamlines at PETRA III

  • Regular Article
  • Published:
The European Physical Journal Plus Aims and scope Submit manuscript

Abstract.

Since 2013, three beamlines for macromolecular crystallography are available to users at the third-generation synchrotron PETRA III in Hamburg: P11, P13 and P14, the latter two operated by EMBL. Beamline P11 is operated by DESY and is equipped with a Pilatus 6M detector. Together with the photon flux of \( 2\times 10^{13}\) ph/s provided by the very brilliant X-ray source of PETRA III, a full data set can be typically collected in less than 2min. P11 provides state-of-the-art microfocusing capabilities with beam sizes down to \( 1\times 1\) μ m2, which makes the beamline ideally suited for investigation of microcrystals and serial crystallography experiments. An automatic sample changer allows fast sample exchange in less than 20s, which enables high-throughput crystallography and fast crystal screening. For sample preparation, an S2 biosafety laboratory is available in close proximity to the beamline.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. H. Franz, O. Leupold, R. Röhlsberger, S.V. Roth, O.H. Seeck, J. Spengler, J. Strempfer, M. Tischer, J. Viefhaus, E. Weckert, T. Wroblewski, Synchrotron Radiat. News 19, 25 (2006)

    Article  Google Scholar 

  2. K. Balewski, W. Brefeld, W. Decking, Y. Li, G.K. Sahoo, R. Wanzenberg, Proceedings of EPAC, Lucerne, Switzerland, PP. 2302--2304

  3. https://door.desy.de/door/index.php

  4. http://photon-science.desy.de/users_area/users_guide/index_eng.html

  5. http://www.biostruct-x.eu/content/apply-funding

  6. http://photon-science.desy.de/facilities/petra_iii/beamlines/p11_bio_imaging_and_diffraction

  7. http://www.embl-hamburg.de/services/mx/P13

  8. http://www.embl-hamburg.de/services/mx/P14

  9. A. Meents, B. Reime, N. Stuebe, P. Fischer, M. Warmer, D. Goeries, J. Roever, J. Meyer, J. Fischer, A. Burkhardt, I. Vartiainen, P. Karvinen, C. David, SPIE Optical Engineering + Applications, Proceedings of SPIE 8851, San Diego, United States, p. 88510K (2013)

  10. I. Vartiainen, M. Warmer, D. Goeries, E. Herker, R. Reimer, C. David, A. Meents, J. Synchrotron Radiat. 21, 790 (2014)

    Article  Google Scholar 

  11. K. Stachnik, I. Mohacsi, I. Vartiainen, N. Stuebe, J. Meyer, M. Warmer, C. David, A. Meents, Appl. Phys. Lett. 107, 011105 (2015)

    Article  ADS  Google Scholar 

  12. http://www.suna-precision.com/

  13. W. Kabsch, Acta Crystallogr. D 66, 125 (2010)

    Article  Google Scholar 

  14. A.G.W. Leslie, Acta Crystallogr. D 62, 48 (2006)

    Article  Google Scholar 

  15. G.M. Sheldrick, Acta Crystallogr. A 64, 112 (2008)

    Article  ADS  Google Scholar 

  16. P.D. Adams, P.V. Afonine, G. Bunkóczi, V.B. Chen, I.W. Davis, N. Echols, J.J. Headd, L.-W. Hung, G.J. Kapral, R.W. Grosse-Kunstleve, A.J. McCoy, N.W. Moriarty, R. Oeffner, R.J. Read, D.C. Richardson, J.S. Richardson, T.C. Terwilliger, P.H. Zwart, Acta Crystallogr. D 66, 213 (2010)

    Article  Google Scholar 

  17. M.D. Winn, C.C. Ballard, K.D. Cowtan, E.J. Dodson, P. Emsley, P.R. Evans, R.M. Keegan, E.B. Krissinel, A.G.W. Leslie, A. McCoy, S.J. McNicholas, G.N. Murshudov, N.S. Pannu, E.A. Potterton, H.R. Powell, R.J. Read, A. Vagin, K.S. Wilson, Acta. Crystallogr. D 67, 235 (2011)

    Article  Google Scholar 

  18. P. Emsley, B. Lohkamp, W.G. Scott, K. Cowtan, Acta Crystallogr. D 66, 486 (2010)

    Article  Google Scholar 

  19. O.V. Dolomanov, L.J. Bourhis, R.J. Gildea, J.A.K. Howard, H. Puschmann, J. Appl. Crystallogr. 42, 339 (2009)

    Article  Google Scholar 

  20. P. Roedig, I. Vartiainen, R. Duman, S. Panneerselvam, N. Stuebe, O. Lorbeer, M. Warmer, G. Sutton, D.I. Stuart, E. Weckert, C. David, A. Wagner, A. Meents, Sci. Rep. 5, 10451 (2015)

    Article  ADS  Google Scholar 

  21. K. Schulte, N. Pawlowski, K. Faelber, C. Fröhlich, J. Howard, O. Daumke, BMC Biol. 14, 14 (2016) DOI:10.1186/s12915-016-0236-7

    Article  Google Scholar 

  22. J. Hellmich, M. Bommer, A. Burkhardt, M. Ibrahim, J. Kern, A. Meents, F. Müh, H. Dobbek, A. Zouni, Structure 22, 1607 (2014)

    Article  Google Scholar 

  23. F. Stellato, D. Oberthür, M. Liang, R. Bean, C. Gati, O. Yefanov, A. Barty, A. Burkhardt, P. Fischer, L. Gallia, R.A. Kirian, J. Meyer, S. Panneerselvam, C.H. Yoona, F. Chervinskii, E. Speller, T.A. White, C. Betzel, A. Meents, H.N. Chapman, IUCrJ 1, 204 (2014)

    Article  Google Scholar 

  24. A. Burkhardt, M. Warmer, S. Panneerselvam, A. Wagner, A. Zouni, C. Glöckner, R. Reimer, H. Hohenberg, A. Meents, Acta Crystallogr. F 68, 495 (2012)

    Article  Google Scholar 

  25. A. Burkhardt, A. Wagner, M. Warmer, R. Reimer, H. Hohenberg, J. Ren, E.E. Fry, D.I. Stuart, A. Meents, Acta Crystallogr. D 69, 308 (2013)

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Alke Meents.

Additional information

Contribution to the Focus Point on “Status of third-generation synchrotron crystallography beamlines: An overview” edited by Gaston Garcia.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Burkhardt, A., Pakendorf, T., Reime, B. et al. Status of the crystallography beamlines at PETRA III. Eur. Phys. J. Plus 131, 56 (2016). https://doi.org/10.1140/epjp/i2016-16056-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1140/epjp/i2016-16056-0

Keywords

Navigation