Abstract.
Since 2013, three beamlines for macromolecular crystallography are available to users at the third-generation synchrotron PETRA III in Hamburg: P11, P13 and P14, the latter two operated by EMBL. Beamline P11 is operated by DESY and is equipped with a Pilatus 6M detector. Together with the photon flux of \( 2\times 10^{13}\) ph/s provided by the very brilliant X-ray source of PETRA III, a full data set can be typically collected in less than 2min. P11 provides state-of-the-art microfocusing capabilities with beam sizes down to \( 1\times 1\) μ m2, which makes the beamline ideally suited for investigation of microcrystals and serial crystallography experiments. An automatic sample changer allows fast sample exchange in less than 20s, which enables high-throughput crystallography and fast crystal screening. For sample preparation, an S2 biosafety laboratory is available in close proximity to the beamline.
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References
H. Franz, O. Leupold, R. Röhlsberger, S.V. Roth, O.H. Seeck, J. Spengler, J. Strempfer, M. Tischer, J. Viefhaus, E. Weckert, T. Wroblewski, Synchrotron Radiat. News 19, 25 (2006)
K. Balewski, W. Brefeld, W. Decking, Y. Li, G.K. Sahoo, R. Wanzenberg, Proceedings of EPAC, Lucerne, Switzerland, PP. 2302--2304
http://photon-science.desy.de/users_area/users_guide/index_eng.html
http://photon-science.desy.de/facilities/petra_iii/beamlines/p11_bio_imaging_and_diffraction
A. Meents, B. Reime, N. Stuebe, P. Fischer, M. Warmer, D. Goeries, J. Roever, J. Meyer, J. Fischer, A. Burkhardt, I. Vartiainen, P. Karvinen, C. David, SPIE Optical Engineering + Applications, Proceedings of SPIE 8851, San Diego, United States, p. 88510K (2013)
I. Vartiainen, M. Warmer, D. Goeries, E. Herker, R. Reimer, C. David, A. Meents, J. Synchrotron Radiat. 21, 790 (2014)
K. Stachnik, I. Mohacsi, I. Vartiainen, N. Stuebe, J. Meyer, M. Warmer, C. David, A. Meents, Appl. Phys. Lett. 107, 011105 (2015)
W. Kabsch, Acta Crystallogr. D 66, 125 (2010)
A.G.W. Leslie, Acta Crystallogr. D 62, 48 (2006)
G.M. Sheldrick, Acta Crystallogr. A 64, 112 (2008)
P.D. Adams, P.V. Afonine, G. Bunkóczi, V.B. Chen, I.W. Davis, N. Echols, J.J. Headd, L.-W. Hung, G.J. Kapral, R.W. Grosse-Kunstleve, A.J. McCoy, N.W. Moriarty, R. Oeffner, R.J. Read, D.C. Richardson, J.S. Richardson, T.C. Terwilliger, P.H. Zwart, Acta Crystallogr. D 66, 213 (2010)
M.D. Winn, C.C. Ballard, K.D. Cowtan, E.J. Dodson, P. Emsley, P.R. Evans, R.M. Keegan, E.B. Krissinel, A.G.W. Leslie, A. McCoy, S.J. McNicholas, G.N. Murshudov, N.S. Pannu, E.A. Potterton, H.R. Powell, R.J. Read, A. Vagin, K.S. Wilson, Acta. Crystallogr. D 67, 235 (2011)
P. Emsley, B. Lohkamp, W.G. Scott, K. Cowtan, Acta Crystallogr. D 66, 486 (2010)
O.V. Dolomanov, L.J. Bourhis, R.J. Gildea, J.A.K. Howard, H. Puschmann, J. Appl. Crystallogr. 42, 339 (2009)
P. Roedig, I. Vartiainen, R. Duman, S. Panneerselvam, N. Stuebe, O. Lorbeer, M. Warmer, G. Sutton, D.I. Stuart, E. Weckert, C. David, A. Wagner, A. Meents, Sci. Rep. 5, 10451 (2015)
K. Schulte, N. Pawlowski, K. Faelber, C. Fröhlich, J. Howard, O. Daumke, BMC Biol. 14, 14 (2016) DOI:10.1186/s12915-016-0236-7
J. Hellmich, M. Bommer, A. Burkhardt, M. Ibrahim, J. Kern, A. Meents, F. Müh, H. Dobbek, A. Zouni, Structure 22, 1607 (2014)
F. Stellato, D. Oberthür, M. Liang, R. Bean, C. Gati, O. Yefanov, A. Barty, A. Burkhardt, P. Fischer, L. Gallia, R.A. Kirian, J. Meyer, S. Panneerselvam, C.H. Yoona, F. Chervinskii, E. Speller, T.A. White, C. Betzel, A. Meents, H.N. Chapman, IUCrJ 1, 204 (2014)
A. Burkhardt, M. Warmer, S. Panneerselvam, A. Wagner, A. Zouni, C. Glöckner, R. Reimer, H. Hohenberg, A. Meents, Acta Crystallogr. F 68, 495 (2012)
A. Burkhardt, A. Wagner, M. Warmer, R. Reimer, H. Hohenberg, J. Ren, E.E. Fry, D.I. Stuart, A. Meents, Acta Crystallogr. D 69, 308 (2013)
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Contribution to the Focus Point on “Status of third-generation synchrotron crystallography beamlines: An overview” edited by Gaston Garcia.
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Burkhardt, A., Pakendorf, T., Reime, B. et al. Status of the crystallography beamlines at PETRA III. Eur. Phys. J. Plus 131, 56 (2016). https://doi.org/10.1140/epjp/i2016-16056-0
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DOI: https://doi.org/10.1140/epjp/i2016-16056-0